Enzyme Composition for Grain Hull Breakdown and Flour Yield

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Solution Overview

Problem

Current methods for enzymatic treatment of grain, pulse, and cereal crops before processing do not effectively increase the output and quality of milled products while maintaining operational efficiency and dietary value.

Innovation Solution

A composition of cellulases, xylanases, oxidoreductases, esterases, and antioxidants is used to break down grain hulls, enhancing the milling process by increasing the permeability of grains, reducing hull content, and improving flour quality and gluten content, which can be incorporated into existing processing routes without additional capital costs.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional enzymatic treatment methods are used, then starch decomposition occurs, but the efficiency of hydrothermal grain treatment before milling does not increase

Engineering Contradiction:
Improvemilling outputVSAvoidtreatment efficiency
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The treatment is divided into multiple sequential enzymatic stages: first cellulases break down cellulose in hulls, then hemicellulases degrade hemicellulose, followed by proteinases and peptidases for protein modification, and finally amylases for starch decomposition. This segmented approach allows each enzyme system to work optimally on specific substrates, significantly improving overall treatment efficiency and milling output compared to single-enzyme methods

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention uses a composite enzyme preparation containing multiple types of enzymes (cellulases, hemicellulases, proteinases, peptidases, and amylases) working together in a coordinated system. This composite enzyme system addresses the limitation of single-enzyme treatments by simultaneously targeting multiple grain components (cellulose, hemicellulose, proteins, and starch), thereby improving both treatment efficiency and milling productivity

Inventive Principle:
Principle #40Composite materials

2Reliability

If more enzymes are used to improve treatment efficiency, then operational costs increase

Engineering Contradiction:
Improvetreatment efficiencyVSAvoidoperational costs
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

Multiple enzyme systems that would individually require separate treatment steps are merged into a single composite enzyme preparation. This combination allows simultaneous action on multiple grain components (cellulose, hemicellulose, proteins, and starch) in one treatment process, improving efficiency without proportionally increasing costs, as the enzymes work synergistically rather than requiring multiple separate enzyme applications

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The composite enzyme preparation serves multiple functions within a single application: cellulases break down cellulose, hemicellulases degrade hemicellulose, proteinases and peptidases modify proteins, and amylases decompose starch. This multi-functional enzyme system replaces what would otherwise require multiple separate treatment steps, reducing operational complexity and costs while improving overall treatment efficiency

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Productivity

If hull content is reduced to increase flour output, then grain structure integrity is compromised

Engineering Contradiction:
Improveflour outputVSAvoidgrain structure
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The enzyme treatment is applied before milling to preliminarily break down the hull structure and weaken bonding between glumes and kernel. Cellulases and hemicellulases specifically target and degrade the cellulose and hemicellulose in hulls, making them more brittle and easier to separate during milling. This preliminary structural modification allows for more efficient hull removal and higher flour output without compromising the quality of the endosperm

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The enzymatic treatment selectively modifies different parts of the grain with different intensities: hulls are extensively degraded by cellulases and hemicellulases to facilitate removal, while the endosperm is gently modified by amylases to improve flour quality. This localized differential treatment allows hull content to be reduced for increased output while preserving the structural integrity and quality of the valuable endosperm portion

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The solution increases the yield of top-grade flour, improves the whiteness and quality of milled products, and enhances the dietary value by reducing heavy metal content and oxidative deterioration, while maintaining operational efficiency and minimizing water consumption.

Implementation Method 1

cellulases (endoglucanase (1,4-β-glucan-4-glucanohydrolase, κΦ 3.2.1.4) and exoglucanases: cellobiohydrolase (1,4-β-D-glucancellobiohydrolase, κΦ 3.2.1.91)

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

xylanases (hemicellulases (endo-1,4-β-xylanase, κΦ 3.2.1.8, and/or α-L-arabinofuranosidase, κΦ 3.2.1.55))

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 3

oxidoreductases, selected from at least catalases, laccases and peroxidases

Methodology Applied
Scientific EffectOxidation: Oxidation

Implementation Method 4

esterases, selected from at least phytases

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 5

antioxidants, selected from the group consisting of ascorbates, tocopherols, polyphenols, natural vegetable extracts, citric acid or ascorbic acid

Methodology Applied
Scientific EffectOxidation prevention: Oxidation

Data Source

PatentEP3297454B1Composition for treatment of grain, pulse and cereal crop grains before processing
Publication Date: 2020.05.13 PETRICHENKO VALERIYA VLADIMIROVNA

AI summary

This invention relates to agriculture, more specifically, to processing of grain, pulse and cereal crop grains, in particular, to methods of their enzymatic treatment before processing to food products (grits, semolina, flakes, flour etc.) with the aim of increasing the output and quality of the processed and milled product.