Immobilization Device for Enzymes Using Conveyor Spraying
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Solution Overview
Problem
There is a lack of devices and methods for the industrial batch production of immobilized proteins, enzymes, or cells, which limits the scalability and efficiency of their immobilization processes.
Innovation Solution
A device comprising a conveyor belt with spraying-kneading components and a squeezing component is used to immobilize proteins, enzymes, or cells on a carrier, involving sequential spraying and kneading of pre-prepared liquids to achieve even distribution and excess liquid removal, enabling industrial batch production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional immobilization methods are used, then proteins/enzymes/cells can be immobilized on carriers, but industrial batch production capability is lacking
Solution Approach 1:
The device segments the immobilization process into distinct functional modules: conveyor belt for carrier transport, multiple spraying components for liquid distribution, kneading components for mixing, and squeezing components for excess liquid removal. Each module performs a specific function, enabling scalable batch production while maintaining process control
Solution Approach 2:
The conveyor belt system enables continuous transport of carriers through the immobilization process, with multiple spraying-kneading-squeezing cycles performed sequentially. This continuous action allows industrial batch production by processing multiple carriers without interruption, significantly improving productivity over batch-wise conventional methods
2Manufacturing precision
If porous organic foam material is used as carrier, then specific surface area increases and specific activity improves, but lack of preparation device limits application
Solution Approach 1:
The device performs preliminary actions by pre-preparing the liquid mixture containing proteins/enzymes/cells and immobilization reagents before contact with the carrier. The spraying components deliver this pre-prepared liquid uniformly onto the porous carrier surface, ensuring consistent immobilization and high specific activity while simplifying the manufacturing process
Solution Approach 2:
The spraying components use hydraulic principles to deliver the pre-prepared liquid mixture onto the carrier surface through controlled spray nozzles. This hydraulic delivery system ensures uniform distribution of the immobilization mixture across the porous carrier, achieving high specific activity while maintaining ease of manufacture through automated liquid delivery
3Manufacturing precision
If multiple spraying and kneading cycles are performed, then even distribution of liquid is achieved, but process time increases
Solution Approach 1:
The conveyor belt system enables multiple spraying-kneading-squeezing cycles to be performed continuously as carriers move through the device. Each carrier undergoes sequential processing without interruption, achieving even liquid distribution through multiple cycles while minimizing total process time through continuous operation rather than batch processing
Solution Approach 2:
The device segments the multiple spraying and kneading operations into separate stationary components through which the moving carrier passes sequentially. This segmentation allows even distribution to be achieved through multiple cycles while the carrier continuously moves forward, preventing time loss that would occur with repeated stopping and starting in batch processes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device facilitates the industrial batch production of immobilized proteins, enzymes, or cells with increased specific activity due to enhanced specific surface area, improving stability and reuse capabilities.
Implementation Method 1
a spraying component that sprays a pre-prepared liquid for the immobilization of proteins, enzymes or cells on the surface of the carrier
Implementation Method 2
a kneading component that distributes the pre-prepared liquid evenly on the surface of the carrier
Implementation Method 3
a squeezing component that removes the excess liquid remaining on the surface of the carrier
Implementation Method 4
With using the porous organic foam material containing open pores as the carrier of immobilization of enzyme/cell, the specific surface area of immobilized enzyme/cell is increased in a large extent
Data Source
AI summary
Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.