Enzyme Variant Detergent Composition for Low-Temperature Cleaning
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Solution Overview
Problem
Automatic dishwashing detergents face challenges with grit formation and filming/spotting on washed items, especially at low temperatures, and there is a need for more eco-friendly and energy-efficient solutions that maintain cleaning and finishing performance.
Innovation Solution
An automatic dishwashing detergent composition featuring a variant protease and low temperature amylase, along with a lipase, anti-redeposition agents, and sulfonated polymers, which prevents grit formation and improves cleaning and finishing results while being environmentally friendly and energy-efficient.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional detergent compositions are used at high temperatures, then cleaning performance is improved, but energy consumption increases and grit formation occurs
Solution Approach 1:
The patent modifies the temperature parameter by developing enzymes with optimal activity at low temperatures (20-40°C). The protease and amylase variants are engineered to maintain high catalytic activity at these reduced temperatures, enabling effective cleaning without requiring high energy input for heating
Solution Approach 2:
The patent creates a composite enzymatic system combining a variant protease with a low-temperature amylase. This composite enzyme preparation synergistically attacks different soil types - proteins and carbohydrates - providing comprehensive cleaning performance at low temperatures that would be insufficient with single enzymes
2Reliability
If high temperatures are used to prevent grit formation, then cleaning effectiveness is improved, but harmful effects on the environment increase
Solution Approach 1:
The patent changes the operational temperature parameter from conventional high temperatures (50-70°C) to low temperatures (20-40°C). The engineered enzymes compensate for the lower temperature through enhanced catalytic efficiency, maintaining cleaning effectiveness while eliminating the need for energy-intensive heating that contributes to environmental harm
3Reliability
If conventional enzymatic systems are used, then cleaning is achieved, but filming and spotting occur on washed items
Solution Approach 1:
The patent addresses different surface qualities by using a protease variant specifically optimized for removing protein-based soils and preventing film formation on metal and glass surfaces. The enzyme's modified active site and substrate specificity allow it to selectively degrade problematic films without leaving residues
Solution Approach 2:
The patent employs a protease variant engineered with specific amino acid substitutions (G116V, S126L, P127Q, S128A) that copy and improve upon the functionality of conventional proteases. These mutations enhance the enzyme's ability to prevent film formation while maintaining protein degradation capability
4Ease of operation
If unit dose detergent forms are used, then convenience is improved, but grit formation is more acute
Solution Approach 1:
The patent applies preliminary action by incorporating anti-redeposition agents and sulfonated polymers into the unit dose formulation before use. These additives are pre-positioned in the detergent to immediately prevent grit formation and soil redeposition when the detergent is dispensed, addressing the grit problem before it can occur during the washing process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition effectively prevents grit formation and achieves excellent cleaning and finishing results, including reduced filming and spotting, while being environmentally friendly and energy-efficient, even at low temperatures.
Implementation Method 1
The composition provides cleaning and finishing benefits across a wide range of temperatures, including low temperatures
Implementation Method 2
an improved enzymatic system comprising an improved protease (a variant protease as described herein below) in combination with a low temperature amylase
Implementation Method 3
The composition effectively prevents grit formation and achieves excellent cleaning and finishing results, including reduced filming and spotting
Data Source
AI summary
An automatic dishwashing detergent composition comprising:a. at least 0.1 mg of active protease per gram of composition, wherein the protease is a variant of a protease that has at least 70% identity with the amino acid sequence of SEQ ID NO:1. wherein said variant comprise variations in one or more of the following positions: 32, 33, 48-54, 58-62, 94-107, 116, 123-133, 150, 152-156, 158-161, 164, 169, 175-186, 197, 198, 203-216 as compared with the protease in SEQ ID NO:1; andb. at least 0.05 mg of an active low temperature amylase per gram of composition.