Stem Cell-Derived Epidermal Progenitor Conditioned Medium

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Solution Overview

Problem

Current cell therapy products face low survival rates after transplantation and immune rejection issues, and the isolation of stem cells from sources like bone marrow and cord blood is invasive or yields low cell amounts, making them unsuitable for widespread clinical application. Additionally, existing conditioned cell media do not effectively promote cell growth or isolate specific proteins.

Innovation Solution

A method is developed to prepare a stem cell-derived epidermal progenitor cell conditioned medium by differentiating stem cells in a medium containing ascorbic acid and hydrocortisone, followed by culturing the differentiated cells to produce and secrete proteins, which are then recovered from the culture medium, increasing the production of specific proteins like cytokines and growth factors.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If stem cells are isolated from bone marrow or adipose tissues, then stem cells can be obtained for therapy, but the isolation requires invasive methods and cell collection is difficult

Engineering Contradiction:

Improvestem cell amountVSAvoidisolation difficulty

Core Design Contradiction:

Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent extracts stem cells from non-invasive sources (umbilical cord and placenta) rather than invasive sources (bone marrow, adipose tissue). This allows obtaining sufficient stem cell quantities without surgical intervention, directly resolving the contradiction between cell amount and isolation difficulty

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses umbilical cord and placenta as intermediary sources that provide abundant stem cells without requiring invasive procedures. These tissues serve as a mediator between the need for large cell quantities and the desire for easy, non-invasive collection

Inventive Principle:

Principle #24Intermediary (Mediator)

2Reliability

If stem cells are isolated from middle-age or old-age patients, then cell collection is obtained, but differentiation potency and proliferation properties are reduced

Engineering Contradiction:

Improvedifferentiation potencyVSAvoidcell quality

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The patent performs cell collection at birth (umbilical cord and placenta) before aging occurs, preserving high differentiation potency and proliferation properties. This preliminary collection avoids the quality degradation that occurs with age-related stem cell isolation

Inventive Principle:

Principle #10Preliminary action

3Ease of operation

If cord blood is used for stem cell collection, then cell collection is easy, but amounts of stem cells are low

Engineering Contradiction:

Improvecollection easeVSAvoidstem cell amount

Core Design Contradiction:

Ease of operationVSQuantity of substance

Solution Approach 1:

The patent combines collection from two sources (umbilical cord and placenta) that are both easily accessible after birth. This merging of collection sites maintains the ease of operation while significantly increasing the total stem cell amount obtained

Inventive Principle:

Principle #5Merging (Combining)

4Reliability

If cell therapy products are transplanted, then treatment is provided, but survival rate after transplantation is low and immune rejections occur

Engineering Contradiction:

Improvetreatment efficacyVSAvoidimmune rejection

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent creates a copy of the stem cell therapeutic effect through conditioned medium. Instead of transplanting living cells that may be rejected, the patent copies the beneficial protein secretion profile into a cell-free medium that can be administered without triggering immune rejection, while maintaining treatment efficacy

Inventive Principle:

Principle #26Copying

5Quantity of substance

If existing conditioned cell media are used, then cell growth promotion is attempted, but specific proteins are not effectively isolated or produced

Engineering Contradiction:

Improveprotein concentrationVSAvoidprotein composition consistency

Core Design Contradiction:

Quantity of substanceVSManufacturing precision

Solution Approach 1:

The patent changes the culture parameters by using stem cells from specific sources (umbilical cord, placenta) and optimizing culture conditions to achieve high and consistent concentrations of specific proteins in the conditioned medium. This resolves the contradiction between protein quantity and composition consistency

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS11773370B2Stem cell-derived skin precursor cell culture medium and preparation method therefor

Publication Date: 2023.10.03 CHA BIO F&C CO LTD

AI summary

Provided are a method of preparing a stem cell-derived epidermal progenitor cell conditioned medium, the method including: differentiating stem cells to stem cell-derived epidermal progenitor cells by culturing the stem cells in a differentiation medium containing ascorbic acid and hydrocortisone; producing a culture of stem cell-derived epidermal progenitor cells by culturing the differentiated stem cell-derived epidermal progenitor cells in a medium; and recovering the stem cell-derived epidermal progenitor cell conditioned medium from the culture of the stem cell-derived epidermal progenitor cells, a stem cell-derived epidermal progenitor cell conditioned medium prepared by the method, and a method of producing a protein from stem cell-derived epidermal progenitor cells, the method including the method of preparing the stem cell-derived epidermal progenitor cell conditioned medium.