Automated method
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Solution Overview
Problem
Current methods for preparing regenerative epidermal suspensions are labor-intensive and prone to user error, lacking effective automation for consistent production.
Innovation Solution
A system comprising a base unit, cartridge, and pestle for automated preparation of regenerative epidermal suspensions, utilizing motors and heating elements to actuate the pestle for vertical, rotational, and grinding forces, with a mortar screen to separate particulates, and well plates for enzyme and buffer solutions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If manual preparation methods are used, then flexibility and adaptability are maintained, but labor intensity increases and user error increases
Solution Approach 1:
The patent replaces manual mechanical operations with an automated motorized pestle system. The motorized pestle performs the mechanical disaggregation and grinding actions automatically, eliminating the need for manual manual manipulation while maintaining the necessary mechanical forces for tissue processing.
Solution Approach 2:
The system is designed to perform its own operations autonomously. The motorized pestle automatically acts on the tissue sample without requiring continuous manual intervention, and the system can complete the entire preparation process independently once initiated.
2Ease of operation
If manual preparation methods are used, then operational simplicity is maintained, but consistency and reliability decrease
Solution Approach 1:
The system incorporates feedback mechanisms through controlled motor operation. The motorized pestle operates with controlled forces and movements that can be monitored and adjusted, ensuring consistent results. The automated control system maintains reliable parameters throughout the preparation process.
Solution Approach 2:
The system controls and optimizes parameters such as motor speed, force application, and processing time to ensure consistent preparation results. By standardizing these parameters through automated control, the system eliminates the variability inherent in manual operations while maintaining operational simplicity.
3Productivity
If automation is introduced, then productivity and consistency improve, but device complexity increases
Solution Approach 1:
The system is divided into distinct functional modules: a motorized pestle assembly, a mortar cup with screen, a well plate for enzyme/buffer delivery, and a control system. This segmentation allows each component to perform its specific function independently, making the overall complex system more manageable and easier to maintain.
Solution Approach 2:
The motorized pestle serves multiple functions including disaggregation, grinding, and mixing. The well plate system can accommodate different enzyme and buffer combinations for various tissue types. This multi-functionality reduces the need for separate specialized devices, managing complexity through versatile components.
4Reliability
If automated motorized pestle is used, then preparation consistency improves, but force control precision requirements increase
Solution Approach 1:
The system replaces manual force application with a motorized pestle that delivers controlled mechanical forces. The motor control system precisely regulates the forces applied during disaggregation and grinding, ensuring consistent results without requiring manual precision.
Solution Approach 2:
The system controls motor parameters such as speed, torque, and duration to precisely regulate the forces applied to the tissue sample. By standardizing these parameters through programmable control, the system achieves consistent preparation results with reduced precision requirements for manual operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates consistent and efficient production of regenerative epidermal suspensions, reducing human error and improving the quality of tissue regeneration processes.
Implementation Method 1
a heating element; (b) a cartridge, comprising (i) a cartridge top cover having an upper cartridge surface
Implementation Method 2
a pestle comprising a pestle head having at least one disaggregation side
Implementation Method 3
a mortar screen (or screen) transversely disposed within the mortar cup (or cup)
Data Source
AI summary
One or more methods for automated preparation of a regenerative epidermal suspension comprises a processor receiving an initiation signal that indicates that a cartridge that has a cover, a cup containing a tissue sample, and a well plate situated beneath the cover that has a first well containing an enzyme solution and a second well containing a buffer solution, has been placed on a sensor, executing a sequence that actuates a tissue disaggregator against the tissue sample in the cup positioned in the first well, raises the cup to an upper position, rotates the well plate to position the second well beneath the cup, lowers the cup to a position within the second well, and actuates the tissue disaggregator against the tissue sample in the second well.