Ergosterol Peroxide Enrichment via Ozone Oxidation and SMB Chromatography
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Solution Overview
Problem
Current methods for extracting and purifying ergosterol peroxide from Ganoderma lucidum spore powder are complex, yield low quantities, involve toxic reagents, and are not suitable for industrial production, with a lack of efficient and cost-effective processes for large-scale production.
Innovation Solution
An industrial-scale enrichment method involving sporoderm-broken Ganoderma lucidum spore powder using percolation or reflux extraction, followed by medium pressure preparative chromatography and simulated moving-bed chromatography, with recrystallization to achieve high purity ergosterol peroxide.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If traditional extraction and purification methods are used, then ergosterol peroxide can be obtained, but the process is complex and yield is low
Solution Approach 1:
The patent changes the chemical parameters of the extraction system by using ozone oxidation to convert ergosterol to ergosterol peroxide, and adjusts chromatographic parameters (mobile phase composition, flow rate, column temperature) to optimize separation efficiency and achieve high purity with simplified processing
Solution Approach 2:
The patent replaces complex mechanical purification operations with chemical oxidation followed by chromatographic separation, substituting multiple mechanical extraction steps with a more efficient chemical transformation and single-stage chromatographic purification system
2Manufacturing precision
If traditional purification methods are used, then ergosterol peroxide can be obtained, but the cost is high and it is not suitable for industrial production
Solution Approach 1:
The patent optimizes chromatographic parameters including mobile phase composition (acetonitrile-water gradients), flow rates, and column temperature to achieve high purity separation at reduced cost and improved scalability for industrial production
Solution Approach 2:
The patent extracts and isolates ergosterol peroxide from the complex fungal metabolite mixture through selective chromatographic separation, taking out the target compound in high purity form while removing impurities, making the process suitable for industrial production
3Manufacturing precision
If traditional extraction methods are used, then ergosterol peroxide can be obtained, but toxic reagents are involved and environmental safety is compromised
Solution Approach 1:
The patent converts the harmful effect of ozone (a toxic gas) into a beneficial chemical transformation by using controlled ozone oxidation to selectively convert ergosterol to ergosterol peroxide, then decomposing excess ozone to oxygen, thereby eliminating the toxic reagent and achieving high purity product with environmental safety
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method increases yield and purity of ergosterol peroxide, reduces costs, and ensures environmental safety by eliminating toxic substances, making it suitable for industrial production with improved operational efficiency and solvent conservation.
Implementation Method 1
performing extraction by percolation or reflux method
Implementation Method 2
performing extraction by percolation or reflux method
Implementation Method 3
purification by medium pressure preparative chromatography
Implementation Method 4
simulated moving-bed chromatography separation system
Implementation Method 5
recrystallization to achieve high purity ergosterol peroxide
Data Source
AI summary
The invention provides an enrichment method of Ergosterol peroxide from sporoderm-broken Ganoderma lucidum spore powder, comprising the steps of preparing crude extract containing Ergosterol peroxide, purification by medium pressure preparative chromatography, purification by simulated moving-bed, and recrystallization. The operation of this invention is simple and stable, with higher yield and low cost, suitable for industrial-scale production.