Method for producing erythrocyte concentrates
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Solution Overview
Problem
Existing methods for producing red blood cell concentrates do not effectively address the storage stability and pathogen inactivation, particularly after UV irradiation, leading to potential hemolysis and quality degradation during storage.
Innovation Solution
A novel additive solution comprising specific components like disodium hydrogen phosphate, adenine, guanosine, sodium chloride, and trisodium citrate, combined with UV irradiation at wavelengths of 300 to 200 nm, is used to produce and store red blood cell concentrates, enhancing storage stability and pathogen inactivation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If UV irradiation is applied to inactivate pathogens in red blood cell concentrates, then pathogen inactivation is improved, but hemolysis and quality degradation occur during storage
Solution Approach 1:
The patent introduces an intermediary substance (riboflavin or other photosensitizers) that mediates the UV irradiation process. This photosensitizer absorbs UV light and transfers energy to pathogens for inactivation while being protected from excessive damage by the additive solution composition, thereby reducing hemolysis. The photosensitizer acts as a bridge between UV irradiation and pathogen inactivation without directly causing harmful effects to red blood cells.
Solution Approach 2:
The patent modifies the chemical parameters of the storage environment by adjusting the additive solution composition (specific pH range 6.8-7.4, specific ionic strength, presence of protective agents) to reduce the damaging effects of UV irradiation on red blood cells. By changing these parameters, the solution creates a protective environment that allows UV irradiation to inactivate pathogens while minimizing hemolysis.
2Stability of the object's composition
If conventional additive solutions are used for storing red blood cell concentrates, then storage stability is maintained, but pathogen inactivation is insufficient
Solution Approach 1:
The patent merges two previously separate functions into one integrated system: the additive solution simultaneously provides storage stability (by maintaining pH, osmolarity, and nutrient supply) and enables pathogen inactivation (by containing photosensitizers that reactivate under UV light). This combination eliminates the need for separate pathogen inactivation steps and maintains both storage quality and safety.
Solution Approach 2:
The additive solution is designed to perform multiple functions: it serves as a storage medium, a protective agent against UV-induced hemolysis, a nutrient source for red blood cells, and a carrier for photosensitizers that enable pathogen inactivation. This multi-functionality resolves the contradiction by making the storage solution itself capable of pathogen inactivation.
3Reliability
If UV irradiation is performed on diluted red blood cell concentrates, then pathogen inactivation efficiency is improved, but concentration and storage stability become problematic
Solution Approach 1:
The patent applies preliminary action by adding photosensitizers to the additive solution before UV irradiation. This ensures that the photosensitizers are already distributed and protected within the solution, enabling effective pathogen inactivation even when the concentrate is diluted. The preliminary preparation of the solution with protective agents and photosensitizers allows subsequent dilution and irradiation to proceed effectively.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution significantly reduces hemolysis and maintains red blood cell quality, as evidenced by lower hemolysis rates, higher ATP content, and better glucose and lactate levels during storage compared to conventional methods.
Implementation Method 1
irradiation with UV light in the range of 300 to 200 nm
Implementation Method 2
The invention relates to a process for producing red blood cell concentrates diluted with an additive solution, comprising the step of irradiation with UV light in the range of 300 to 200 nm
Data Source
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AI summary
The invention relates to an additive solution for storing erythrocyte concentrates, to erythrocyte concentrates that are provided with the additive solution, and to a process for producing erythrocyte concentrates diluted with the additive solution, comprising the step of irradiation with UV light, and to the use of the additive solution for storing erythrocyte concentrates.