Erythrocyte Simulating Particle Stabilization via Halogen Oxygen Acid Salt Oxidation
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Solution Overview
Problem
Conventional methods for preparing erythrocyte simulating particles for blood cell analyzers face challenges such as environmental pollution due to the use of dichromate and instability in cell morphology and volume over time.
Innovation Solution
A method involving oxidization and fixation treatments using a halogen oxygen acid salt and an aldehyde or acid fixation agent, respectively, to stabilize erythrocytes, thereby creating environmentally friendly and stable erythrocyte simulating particles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If dichromate is used for fixation, then erythrocyte simulating particles can be prepared, but environmental pollution occurs
Solution Approach 1:
The patent changes the chemical composition parameters of the fixation solution by replacing dichromate with aldehyde (such as formaldehyde or glutaraldehyde) combined with potassium permanganate. This substitution maintains the fixation effectiveness while eliminating the environmental pollution caused by dichromate, thus resolving the contradiction between reliability and harmful factors.
Solution Approach 2:
The patent converts the potentially harmful oxidation process into a beneficial fixation mechanism by using potassium permanganate in controlled conditions. The oxidation capability that could be harmful is harnessed to achieve stable fixation of erythrocytes, transforming a potential harm into a benefit while avoiding the environmental issues of dichromate.
2Manufacturing precision
If conventional fixation methods are used, then erythrocyte simulating particles can be prepared, but cell volume changes over time
Solution Approach 1:
The patent uses a composite fixation approach combining aldehyde (formaldehyde or glutaraldehyde) with potassium permanganate. This composite chemical system provides superior stabilization of erythrocyte morphology and volume compared to conventional single-agent fixation methods. The aldehyde provides cross-linking fixation while potassium permanganate ensures oxidation-based structural stability, together preventing volume changes over time.
Solution Approach 2:
The patent employs potassium permanganate, a strong oxidant, to achieve rapid and complete oxidation fixation of erythrocytes. This accelerated oxidation process ensures thorough stabilization of cell structures, preventing subsequent volume changes and morphological alterations that occur with weaker or conventional fixation methods.
3Stability of the object's composition
If aldehyde and dichromate are used together, then erythrocyte stability is improved, but environmental pollution increases
Solution Approach 1:
The patent extracts and removes dichromate from the fixation solution composition, retaining only aldehyde and potassium permanganate. This extraction eliminates the environmentally harmful dichromate while preserving the stabilizing effects of aldehyde and adding the benefits of potassium permanganate-based oxidation fixation.
Solution Approach 2:
The patent changes the chemical composition parameters by substituting dichromate with potassium permanganate. This parameter change maintains or improves fixation effectiveness and stability while eliminating the environmental pollution associated with dichromate, thus resolving the contradiction between stability and harmful factors.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves stable erythrocyte simulating particles that maintain their morphology and volume over an extended period without using heavy metals, thus providing an environmentally friendly solution for quality control and calibration in blood cell analyzers.
Implementation Method 1
performing oxidization and fixation treatments on erythrocytes by using an erythrocyte treatment solution
Implementation Method 2
at least one fixation agent selected from aldehydes or acids
Implementation Method 3
an osmotic pressure of 300-800 Osm/kg·H2O
Data Source
AI summary
Erythrocyte simulating particles, a preparation method therefor, and a quality control or calibrator containing the erythrocyte simulated particles. The method for preparing erythrocyte simulating particles includes: performing oxidization and fixation treatments on erythrocytes by using an erythrocyte treatment solution that has a pH of 5.0-9.0 and an osmotic pressure of 300-800 Osm/kg·H2O, wherein the erythrocyte treatment solution contains at least one oxidization agent selected from halogen oxygen acid salts and at least one fixation agent selected from aldehydes or acids; and washing the erythrocytes treated by the erythrocyte treatment solution and suspending the same in a preservation solution. The described method uses an environmentally friendly halogen oxygen acid salt as an oxidant, to obtain erythrocyte simulating particles that can be stored stably for a long time and have properties similar to natural erythrocyte particles in an erythrocyte detection channel.