Spectrophotometric ESR Measurement Using Angled Cuvette

The patent replaces the traditional mechanical/optical detection system with a spectrophotometric measurement system. The spectrophotometer measures absorbance changes at specific wavelengths (e.g., 540 nm, 570 nm, 630 nm) to detect red blood cell sedimentation, enabling rapid ESR determination within seconds while maintaining measurement accuracy through wavelength-specific absorbance detection of hemoglobin.

The patent changes the measurement parameter from direct visual or optical detection of sedimentation distance to spectrophotometric detection of hemoglobin absorbance changes. By monitoring absorbance variations at multiple wavelengths over time, the system calculates ESR based on the rate of change in optical properties, dramatically reducing measurement time while preserving accuracy.

The patent implements a multi-functional measurement system where a single spectrophotometer performs both hemoglobin concentration measurement and ESR determination using the same blood sample container. The system measures absorbance at multiple wavelengths (e.g., 540 nm, 570 nm, 630 nm) to simultaneously derive hemoglobin values and monitor sedimentation kinetics, eliminating the need for separate containers and procedures.

The patent merges the hemoglobin measurement function and ESR measurement function into a single integrated system. Both measurements are performed on the same blood sample in the same container using the same spectrophotometric detection mechanism, simplifying the workflow and reducing operational complexity while maintaining measurement accuracy through wavelength-specific detection.

The patent implements continuous spectrophotometric monitoring of the blood sample during sedimentation. The system takes multiple absorbance measurements at predetermined time intervals (e.g., every few seconds) and uses kinetic analysis to calculate ESR. This continuous monitoring approach enables rapid measurement completion while maintaining precision through multiple data points and statistical analysis of the sedimentation curve.

The system incorporates feedback mechanisms where measured absorbance values are continuously processed to calculate sedimentation rate. The processor uses the rate of change in absorbance over time, combined with known optical properties of hemoglobin, to dynamically determine ESR. This feedback-driven calculation ensures accurate results even with reduced measurement time by adapting to the actual sedimentation kinetics observed.