Ester Ether Surfactant Stabilizes Hemoglobin Chromatography
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current preparation solutions for hemoglobin analysis in liquid chromatography are unstable, especially when used with lyophilized samples, leading to interference peaks and inaccurate measurements due to the presence of ether-type nonionic surfactants, which affect the stability and accuracy of HbA1c concentration.
Innovation Solution
A preparation solution containing ester ether-type nonionic surfactants, such as polyoxyethylene sorbitan monolaurate, is used to stabilize hemoglobin samples by removing blood plasma components and adding saccharides or antiseptics, ensuring stability and accuracy in HbA1c measurement without interfering peaks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If ether-type nonionic surfactants are used in preparation solution, then hemoglobin samples can be prepared, but new interference peaks appear between HbA1c and HbA0 and HbA1c concentration changes due to poor stability
Solution Approach 1:
The patent changes the chemical structure parameter of the nonionic surfactant from ether-type to ester ether-type. This structural modification eliminates the formation of interference peaks while maintaining the surfactant's ability to stabilize hemoglobin samples during preparation, thus resolving the contradiction between ease of operation and measurement precision.
2Ease of operation
If ether-type nonionic surfactants are used in preparation solution, then hemoglobin samples can be prepared, but HbA1c concentration changes because of poor stability after preparing
Solution Approach 1:
The patent modifies the chemical structure parameter of the nonionic surfactant from ether-type to ester ether-type. This structural change enhances the stability of HbA1c concentration after sample preparation by preventing unwanted interactions between the surfactant and hemoglobin components, thereby resolving the contradiction between ease of operation and compositional stability.
3Adaptability or versatility
If a universal preparation solution is used for all hemoglobin samples including lyophilized samples, then sample preparation is simplified, but interference peaks appear and measurement accuracy deteriorates
Solution Approach 1:
The patent changes the chemical structure parameter of the nonionic surfactant to ester ether-type, which maintains compatibility across different sample types (blood samples, lyophilized samples, frozen samples) while eliminating interference peak formation. This resolves the contradiction between adaptability and measurement precision by finding a surfactant structure that works universally without causing measurement artifacts.
Data Source
Figure 1(A)~1(B)
Figure 2(A)~2(B)
Figure 3(A)~3(C)
AI summary
The present invention provides a preparation solution for analyzing hemoglobin in a blood sample and a liquid chromatography method for measuring hemoglobins using the preparation. The object is achieved by providing a hemoglobin preparation solution for measuring hemoglobins from a hemoglobin-containing sample. The hemoglobin preparation solution contains 0.01 to 0.4 wt% of at least one ester ether-type nonionic surfactant and does not contain an ether-type nonionic surfactant.