Extracellular Vesicle Recovery via Binding Complexation
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Solution Overview
Problem
Current methods for recovering target entities, such as extracellular vesicles, from biological samples are inefficient due to the presence of contaminants, which can interfere with the recovery process and reduce the purity and yield of target entities.
Innovation Solution
A method involving the use of binding compositions with specific moieties that interact with recognition motifs on the target entities to form a complexed population, altering their physical properties, allowing for selective recovery and fractionation from contaminants, using devices with porous units and chambers to facilitate this process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional isolation methods (differential ultracentrifugation, polymer precipitation, size exclusion) are used to recover target entities, then recovery is achieved, but the presence of contaminants reduces purity and yield
Solution Approach 1:
The patent uses binding compositions as intermediary agents that specifically bind to recognition motifs on target entities (such as EVs). These binding compositions act as mediators between the target entities and the separation process, enabling selective recovery while excluding contaminants. The binding compositions form complexes with target entities that have altered physical properties, facilitating purification without loss of yield.
2Manufacturing precision
If extensive sample clearing is performed to remove contaminants, then purity of target entities improves, but the process becomes more complex and time-consuming
Solution Approach 1:
The patent extracts only the necessary binding compositions from complex mixtures, rather than performing extensive clearing of the entire sample. The binding compositions are selected to specifically recognize and bind to target entities, allowing direct recovery from relatively crude samples without extensive preprocessing steps.
Solution Approach 2:
The patent changes the physical parameters of target entities by forming complexes with binding compositions. This alters properties such as size, density, or charge, enabling simple separation steps to achieve high purity without complex multi-step clearing procedures.
3Productivity
If multiple components are added to samples to effect target entity recovery, then recovery efficiency improves, but the complexity of the method increases
Solution Approach 1:
The binding compositions are designed to perform multiple functions: they specifically recognize and bind to target entities, alter physical properties for separation, and enable concentration. This multi-functionality reduces the need for multiple separate components or reagents in the recovery process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables rapid and selective recovery of target entities with improved purity and yield, even in the presence of contaminants, by altering their physical properties for separation and reducing the need for extensive sample clearing.
Implementation Method 1
contacting the sample with a plurality of binding compositions, each binding composition having first and/or second moieties capable of binding with a recognition motif of the target entities under conditions to allow complexing the target entities with the plurality of binding compositions to form a complexed population of target entities
Data Source
AI summary
A device for recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described.