Extracellular Vesicle Concentration via Low Molecular Weight Polyether
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
The concentration of extracellular vesicles in liquid samples is generally low, making it challenging to detect them or their biomarkers effectively using existing methods.
Innovation Solution
A method involving two-phase partitioning using a polysaccharide and a polyether with an average molecular weight of 20,000 or less, which separates the mixture into a first fraction with a higher concentration of extracellular vesicles, allowing for efficient concentration and collection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional two-phase partitioning methods using polyethylene glycol and dextran are used, then extracellular vesicles can be collected, but the collection rate is insufficient and the concentration is low
Solution Approach 1:
The patent changes the molecular weight parameter of the polyether from conventional high molecular weight (polyethylene glycol) to low molecular weight (20,000 or less), which fundamentally alters the phase partitioning behavior and dramatically improves both collection rate and concentration of extracellular vesicles
Solution Approach 2:
The patent creates a composite system by combining polysaccharide and low molecular weight polyether, where the two materials work synergistically to achieve superior separation and concentration effects that neither material could achieve alone
2Productivity
If polymeric precipitation kits are used, then extracellular vesicles can be precipitated and collected, but the operational efficiency and reproducibility are insufficient
Solution Approach 1:
The patent optimizes the molecular weight parameter of polyether to 20,000 or less, which creates optimal phase partitioning conditions that simultaneously improve operational efficiency and ensure reproducible results across different experiments
Solution Approach 2:
The patent creates distinct local environments in the two phases through the polysaccharide-polyether system, where the first phase provides optimal conditions for extracellular vesicle concentration with high reproducibility and efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves a high collection rate of extracellular vesicles, improving the detection of these vesicles and their biomarkers, with enhanced operational efficiency and reproducibility compared to existing techniques.
Implementation Method 1
two-phase partitioning using a polysaccharide and a polyether having a relatively low molecular weight
Implementation Method 2
centrifuging the mixture
Data Source
AI summary
Disclosed is a method for concentrating extracellular vesicles, comprising preparing a mixture comprising a first fraction and a second fraction by mixing a liquid sample comprising extracellular vesicles, a polysaccharide, and a polyether having an average molecular weight of 20,000 or less, wherein the first fraction comprises a higher concentration of extracellular vesicles than the second fraction, and the first fraction comprises a higher concentration of extracellular vesicles than the liquid sample.