Extracellular Vesicle Preservation Solution Formulation
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Solution Overview
Problem
Extracellular vesicles/exosomes are prone to degradation after purification, making long-term preservation challenging and hindering industrial development due to extended process durations and susceptibility to modification or drug conjugation.
Innovation Solution
A preservation solution comprising a carrier solvent such as polysorbate 80, sucrose, or polyethylene glycol 3350/4000, and a stabilizer like salts or amino acids, with a pH range of 5 to 7.4, is used to create appropriate preservation conditions for extracellular vesicles/exosomes, enabling long-term preservation in frozen environments through gradual cooling and potential drying.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If extracellular vesicles/exosomes are purified and stored without specific preservation solution, then the purification process is simple and quick, but the vesicles degrade rapidly and cannot be preserved for long-term use
Solution Approach 1:
The patent changes the chemical parameters of the preservation environment by formulating a specific solution with controlled pH (5-7.4), osmolarity (200-400 mOsm/L), and specific composition including carrier solvents (polysorbate 80, sucrose, PEG-3350/4000) and stabilizers (salts, amino acids). These parameter changes create optimal conditions that prevent vesicle degradation and enable long-term preservation while maintaining vesicle integrity and biological activity.
Solution Approach 2:
The preservation solution acts as an intermediary medium between the purified vesicles and the storage environment. It provides a protective chemical environment that mediates the interaction between vesicles and external factors, preventing direct exposure to harmful conditions while maintaining vesicle stability during long-term storage.
2Productivity
If extracellular vesicles/exosomes are purified in large quantities for industrial development, then the productivity increases, but the complexity of preservation and degradation control increases
Solution Approach 1:
The preservation solution is designed as a universal formulation that can be applied to various types of extracellular vesicles and exosomes from different sources. The standardized composition with specific pH range, osmolarity, and component ratios provides a single multi-functional preservation system that handles large-scale purification and storage requirements without requiring source-specific complex formulations.
3Adaptability or versatility
If modification or drug conjugation is performed on purified extracellular vesicles/exosomes, then the functional application value increases, but the process duration extends and preservation challenges increase
Solution Approach 1:
The preservation solution is prepared in advance with all necessary stabilizing components already incorporated at optimal concentrations. This preliminary preparation of the preservation environment allows for rapid subsequent modification and drug conjugation processes without requiring time-consuming preservation adjustments, thereby reducing overall process duration while maintaining high functional application value.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution maintains over 80% concentration and biological activity of extracellular vesicles/exosomes for several weeks, facilitating their use in medical research and applications by ensuring efficient preservation in both liquid and dry forms, thus enhancing time efficiency and commercial value.
Implementation Method 1
a stabilizer selected from the group consisting of salts, amino acids, and amino acid salts
Implementation Method 2
gradually cooling the mixed preservation solution and placing it in a frozen preservation environment
Data Source
AI summary
A solution for preserving extracellular vesicles/exosomes has a carrier solvent selected from a group consisting of polysorbate 80, sucrose, and polyethylene glycol 3350/4000; and a stabilizer selected from a group consisting of salts, amino acids, and amino acid salts; wherein the pH of the preservation solution ranges from 5 to 7.4.