Ex Vivo Organ Purification Kit with Fractionation Filter
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Solution Overview
Problem
Existing devices for ex vivo lung perfusion do not effectively eliminate cell waste, cell elements, and inflammatory substances from explanted organs, leading to organ dysfunction and transplant failure.
Innovation Solution
A kit comprising a fractionation filter with a cut-off range of 80,000 to 800,000 Da and an absorption cartridge, along with fluid connection means, is used to purify explanted organs by retaining cell fragments and inflammatory substances, allowing cytokines to pass through, thereby conditioning the organs for reimplantation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional EVLP devices are used to perfuse explanted organs, then the organs can be temporarily maintained, but cell waste, cell elements and inflammatory substances are not eliminated, leading to organ dysfunction
Solution Approach 1:
The invention extracts and removes harmful substances (inflammatory cytokines, cell elements, and waste products) from the perfusion liquid through a specialized filter system. The filter selectively retains these harmful factors while allowing beneficial substances to pass, thereby eliminating the source of organ dysfunction and improving transplant reliability.
Solution Approach 2:
The filter acts as an intermediary component between the perfusion liquid and the organ. It mediates the interaction by selectively allowing certain substances to pass while blocking harmful factors, thus protecting the organ from inflammatory damage while maintaining necessary perfusion functions.
2Reliability
If no filtration system is used during EVLP, then the device complexity is low, but harmful inflammatory substances accumulate and cause organ deterioration
Solution Approach 1:
The invention employs porous filter materials with specific pore sizes and molecular weight cut-offs (80,000-800,000 Da) to selectively retain inflammatory substances and cell elements. The porous structure allows the filter to capture harmful factors while maintaining fluid flow and not interfering with essential perfusion functions, achieving purification without excessive complexity.
3Object-generated harmful factors
If a filtration system with high retention capability is used, then harmful substances are eliminated, but the device complexity and operational difficulty increase
Solution Approach 1:
The invention combines multiple filtration functions (size-based filtration, molecular weight-based filtration, and adsorption) into a single integrated filter unit. This merging of functions allows the device to eliminate harmful substances effectively while maintaining ease of operation, as the user only needs to connect the single filter to the perfusion circuit without managing multiple separate filtration components.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit effectively removes cytokines, inflammation mediators, and cell elements, improving organ function and increasing the success rate of transplants by eliminating harmful substances that cause organ deterioration.
Implementation Method 1
a separation means comprising at least one fractionation filter and an absorption cartridge (5), in which said fractionation filter has a cut-off in the range from 80,000 to 800,000 Da
Implementation Method 2
an absorption cartridge (5), in which said fractionation filter has a cut-off in the range from 80,000 to 800,000 Da and is positioned upstream of said absorption cartridge
Data Source
Figure 1
Figure 2
AI summary
The present invention concerns a kit (1) for ex vivo conditioning and purification of an explanted organ comprising: connection means from and to said organ (12); fluid connection means (2); a fractionation filter (4) and an absorption cartridge (5), in which the fractionation filter (4) has a cut-off ranging from 80,000 to 800,000 Da and is positioned upstream of said absorption cartridge (5). The kit advantageously further comprises a pump (3). A plasma filter with a pore diameter from 0.2 to 0.6 micron may be provided upstream of the fractionation filter in order to prevent clogging thereof.