Exiguobacterium indicum YAN2 Biosynthesis of Nano-Selenium
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Solution Overview
Problem
Current methods for selenium supplementation, particularly in selenium-deficient regions, face challenges such as narrow effective range, potential for selenium poisoning, and limitations in bioavailability and safety compared to inorganic forms.
Innovation Solution
The use of a strain of Exiguobacterium indicum (E. indicum YAN2) for the biosynthesis of nano-selenium through fermentation, which produces spherical biosynthetic nano-selenium (Bio-SeNP) with enhanced bioavailability and safety.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If inorganic selenium supplements are used, then selenium deficiency can be addressed, but the effective range is extremely narrow and excessive selenium may cause poisoning
Solution Approach 1:
The patent changes the physical and chemical parameters of selenium from inorganic forms to nano-scale elemental selenium particles with controlled size (20-500 nm). This parameter change transforms selenium into a form that is more easily absorbed by the body while reducing toxicity, thus expanding the effective range and reducing poisoning risk
Solution Approach 2:
The patent introduces microorganisms (such as Aspergillus niger, Trichoderma harzianum, and Paecilomyces variabilis) as intermediary agents that facilitate the transformation of inorganic selenium compounds into nano-selenium. These microorganisms act as mediators that convert toxic inorganic forms into bioavailable nano-form without causing poisoning
2Quantity of substance
If chemical synthesis methods are used to produce nano-selenium, then nano-selenium can be obtained, but the process causes pollution and has high energy consumption
Solution Approach 1:
The patent replaces chemical synthesis methods with biological synthesis methods using microorganisms. Instead of using chemical reagents and high-energy processes, the invention employs microbial metabolism to transform inorganic selenium compounds into nano-selenium through enzymatic reactions, thereby eliminating pollution and reducing energy consumption
Solution Approach 2:
The patent utilizes the natural metabolic capabilities of microorganisms to synthesize nano-selenium. The microorganisms self-transform selenium compounds into nano-form through their inherent enzymatic systems, eliminating the need for external chemical additives and high-energy input processes
3Use of energy by stationary object
If conventional microbial synthesis methods are used, then nano-selenium can be produced with low energy consumption, but the habitats of the strains are relatively narrow limiting application development
Solution Approach 1:
The patent selects and employs microorganisms that possess broad environmental adaptability and can thrive in diverse habitats. The selected strains (Aspergillus niger, Trichoderma harzianum, Paecilomyces variabilis) are known for their versatility in different environmental conditions, allowing them to produce nano-selenium across various geographical locations and environmental settings
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The nano-selenium produced by E. indicum YAN2 significantly increases selenium content in crops, improves crop quality, and enhances stress resistance, while being environmentally friendly and safe for application.
Implementation Method 1
Some of the microorganisms can also transform highly toxic selenite or selenate into red elemental nano-selenium
Implementation Method 2
a method for synthesizing nano-selenium by fermenting the strain
Data Source
AI summary
An Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.