Exogenous Polynucleotides for Nitrogen Use Efficiency and Yield
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Solution Overview
Problem
Current agricultural practices face challenges in increasing nitrogen use efficiency in plants, leading to high fertilizer input costs and environmental pollution, while also dealing with abiotic stresses like drought, salinity, and temperature fluctuations, which affect crop yields and quality.
Innovation Solution
Expression of novel exogenous polynucleotides and polypeptides within plants, specifically those with nucleic acid sequences at least 80% identical to certain SEQ IDs, to enhance nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, and abiotic stress tolerance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If inorganic nitrogenous fertilizers are applied to increase crop yield, then plant growth and biomass are improved, but nitrogen use efficiency decreases and environmental pollution increases
Solution Approach 1:
The patent modifies plant genetic parameters by introducing and expressing exogenous polynucleotides that encode polypeptides involved in nitrogen metabolism. This changes the plant's physiological parameters to improve nitrogen uptake, assimilation, and utilization efficiency, allowing the plant to achieve higher yields with reduced fertilizer input.
Solution Approach 2:
The patent enables plants to self-enhance their nitrogen use efficiency through genetic modification. The introduced polynucleotides allow plants to autonomously improve their nitrogen metabolism pathways, reducing dependence on external fertilizer applications and minimizing nitrogen loss to the environment.
2Productivity
If inorganic nitrogenous fertilizers are applied to increase crop yield, then plant growth is improved, but environmental pollution increases
Solution Approach 1:
The patent changes the plant's physiological parameters for nitrogen metabolism by introducing exogenous polynucleotides. This modification improves the plant's ability to utilize nitrogen efficiently, thereby reducing excess nitrogen that would otherwise be lost to the environment as pollution.
3Loss of energy
If exogenous polynucleotides are expressed to increase nitrogen use efficiency, then fertilizer requirements are reduced, but genetic modification complexity increases
Solution Approach 1:
The patent extracts and utilizes specific functional polynucleotides and polypeptides that are responsible for nitrogen metabolism enhancement. By isolating and expressing only the essential genetic elements needed for nitrogen use efficiency improvement, the approach simplifies the genetic modification process while achieving the desired reduction in fertilizer requirements.
Data Source
AI summary
Provided are methods of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO:1-467, 785-3047; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO:468-784, 3048-4333, 4335-4682. Also provided isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1-467, 785-3047, which can be used to increase nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant.