Exosomal TβRII Protein Detection for Non-Invasive Breast Cancer Diagnosis
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Solution Overview
Problem
Current breast cancer detection methods require tumor tissue samples, which are difficult to obtain for some patients, and are invasive, making it challenging to detect metastasis effectively.
Innovation Solution
A breast cancer detection kit utilizing exosomal TβRII protein as a marker in peripheral blood, using direct-labeled primary antibodies and flow cytometry for qualitative or quantitative detection, allowing for non-invasive diagnosis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If immunohistochemical technique or Western blot is used to detect breast cancer metastasis, then detection specificity is improved, but the need for tumor tissue acquisition increases operational complexity and invasiveness
Solution Approach 1:
The invention extracts the detection target from solid tumor tissue to liquid exosomes in body fluids. By detecting TβRII protein specifically on exosome surfaces using flow cytometry with fluorescently labeled antibodies, the method obtains cancer-related information from easily accessible liquid samples while maintaining high detection specificity
Solution Approach 2:
The invention uses exosomes as intermediary carriers that transport TβRII protein from tumor cells to body fluids. These exosomes serve as mediators between the tumor and the detection system, allowing indirect but accurate detection of tumor metastasis through liquid biopsies without direct tissue sampling
2Reliability
If tumor tissue samples are collected for metastasis detection, then diagnostic accuracy is improved, but patient discomfort and procedural invasiveness increase
Solution Approach 1:
The invention extracts diagnostic information from invasive tissue biopsies and relocates it to non-invasive liquid exosome samples. By detecting TβRII on exosomes in blood or other body fluids, the method achieves the same diagnostic reliability without the harm of surgical tissue collection
Solution Approach 2:
The invention uses exosomes as natural copies or proxies of tumor cells. These exosomes carry characteristic TβRII markers that replicate the diagnostic information found in tumor tissues, allowing detection of metastasis through liquid copies rather than solid tissue originals
3Reliability
If multiple samples are collected to account for random tumor metastasis, then detection comprehensiveness is improved, but sample processing complexity and time consumption increase
Solution Approach 1:
The invention merges multiple detection targets into a single unified assay by detecting TβRII protein on exosomes. This single marker detection on liquid exosomes simultaneously provides comprehensive metastasis information that would otherwise require multiple separate tissue samples and analyses
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit enables accurate prediction and monitoring of breast cancer development and metastasis with high sensitivity and specificity, offering a minimally invasive 'liquid biopsy' approach, suitable for large-scale screening without the need for organic solvents or toxic reagents.
Implementation Method 1
a direct-labeled primary antibody against TβRII protein
Implementation Method 2
flow cytometry for qualitative or quantitative detection
Implementation Method 3
detecting the label on the direct standard primary antibody
Data Source
AI summary
The present invention discloses an application of the exosome TβRII protein as a marker in preparing a breast cancer diagnostic kit, and belongs to the technology field of breast cancer detection kits. The present invention finds that the content of TβRII positive exosomes in the serum of breast cancer patients is significantly higher than these of healthy people, and after the surgery, the content of TβRII exosomes in the serum of breast cancer patients reduces significantly, which suggests the value of the TβRII positive exosomes during the diagnosis and prognosis of breast cancer. The present invention provides a breast cancer diagnosis kit based on detecting exosome TβRII protein in peripheral blood, which is simple in operation, and only needs to extract a small amount of peripheral blood of the test population, thereby detecting whether the test population has breast cancer and evaluating the tumor of the patient. The degree of malignancy and the state of metastasis.