Exosome Extraction System Continuous Processing
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Solution Overview
Problem
Current methods for exosome extraction are complex and yield low production due to separate processes for cell culture and separation, resulting in inefficient exosome recovery.
Innovation Solution
An exosome extraction system that includes a raw material supply unit, a cell disruptor to convert cell fluid into a lysate, a filter unit with primary and secondary filters for size-based separation, and a recovery unit to collect exosomes, utilizing tangential flow filtration and size exclusion chromatography for efficient separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If separate centrifuge process is used after cell culture, then exosomes can be separated, but the process becomes complicated and production yield decreases
Solution Approach 1:
The patent combines cell culture and exosome separation into a single integrated system. The cell culture chamber and centrifuge are merged into one device, allowing cells to be cultured and exosomes to be separated simultaneously without requiring separate processing steps. This integration eliminates the complexity of sequential operations and improves production yield by continuous processing.
Solution Approach 2:
The invention enables continuous exosome extraction throughout the cell culture period rather than performing separation only after culture completion. The centrifuge operates continuously or periodically during cell proliferation, maintaining continuous useful action to extract exosomes as they are released, thereby increasing overall production yield and eliminating process complexity.
2Productivity
If traditional centrifugation method is used, then exosomes can be obtained, but the process is time-consuming and yield is low
Solution Approach 1:
The patent implements continuous centrifugation during cell culture, allowing exosomes to be extracted continuously as cells proliferate and release exosomes. This continuous processing eliminates idle time between culture and separation steps, significantly reducing processing time and improving productivity compared to batch processing methods.
Solution Approach 2:
The system performs preliminary centrifugation steps during the cell culture period rather than waiting for culture completion. By initiating separation processes early and continuously during cell proliferation, the system reduces overall processing time and increases productivity through overlapping of culture and extraction operations.
Data Source
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AI summary
An exosome extraction system is provided. The exosome extraction system according to one embodiment of the present invention may comprise: a raw material supply unit for supplying a cell fluid containing cells; a cell disruptor which disrupts the cells through physical impact to convert the cell fluid supplied from the raw material supply unit, into a cell lysate including microvesicles, exosomes, and proteins; a filter unit for separating the exosomes from the cell lysate supplied from the cell disruptor; and a recovery unit for recovering the exosomes separated from the filter unit.