Exosome Processing via Magnetic Binding Agents
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Solution Overview
Problem
Current methods for isolating exosomes are limited by the expense and inefficiency of standard sorting technologies, such as flow activated sorting, and the challenges of magnetic separation, which can lead to reagent clumping and off-target binding, making it difficult to selectively remove desired and undesired components for therapeutic applications like skin rejuvenation or cosmetic uses.
Innovation Solution
A method and system for processing exosomes that involves using binding agents, such as antibodies or oligonucleotides, labeled with magnetic or optical particles to selectively capture and release exosome subpopulations through magnetic or electric fields, allowing for the enrichment of desired exosome subpopulations from mixed populations in biological samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If flow activated sorting is used to isolate exosomes, then sorting capability is improved, but cost and time efficiency deteriorate
Solution Approach 1:
The patent extracts and removes unwanted exosome subpopulations from the mixed population using binding agents that specifically target and eliminate harmful exosomes, rather than attempting to sort all exosomes individually. This extraction approach achieves the desired purification without the high costs and time requirements of comprehensive flow sorting.
Solution Approach 2:
The patent introduces binding agents as intermediary substances that mediate between the mixed exosome population and the separation process. These binding agents specifically bind to unwanted exosome subpopulations, enabling selective removal without requiring direct observation or sorting of each exosome, thus improving efficiency while maintaining sorting capability.
2Productivity
If magnetic separation is used to remove exosome subpopulations, then sorting efficiency is improved, but reagent clumping and off-target binding occur
Solution Approach 1:
The patent applies local quality by using binding agents with highly specific local binding characteristics that target only particular exosome subpopulations. This specificity prevents off-target binding and reagent clumping, as each binding agent is designed to recognize and bind only to specific markers on the intended target exosomes, maintaining reagent stability and sorting efficiency.
3Ease of manufacture
If standard filtration is used to isolate exosomes, then isolation simplicity is improved, but selective removal of subpopulations deteriorates
Solution Approach 1:
The patent introduces binding agents as intermediary substances that enable selective removal of unwanted exosome subpopulations after initial isolation. This two-step approach maintains the simplicity of filtration for initial isolation while adding specificity through binding agents that selectively bind to and remove harmful subpopulations, achieving both ease of manufacture and subpopulation selectivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables a cost- and time-efficient method for selectively removing exosome subpopulations, enhancing the therapeutic potential of exosomes by isolating specific subpopulations for pharmaceutical or cosmetic applications, improving the purity and effectiveness of exosome-based treatments.
Implementation Method 1
labeled with magnetic or optical particles to selectively capture and release exosome subpopulations through magnetic or electric fields
Implementation Method 2
labeled with magnetic or optical particles to selectively capture and release exosome subpopulations through magnetic or electric fields
Data Source
AI summary
Methods and systems of processing exosomes from a biologic sample, including providing a biological sample having a mixed population of exosomes, wherein the mixed population of exosomes includes two or more distinct subpopulations of exosomes; processing the biological sample to selectively remove one or more exosome subpopulations from the mixed population of exosomes thereby obtaining a sample enriched with a desired subpopulation of exosomes; and adding the enriched sample of exosomes to a pharmaceutically acceptable carrier.