Exosome-Stem Cell Medium with Botulinum Toxin for Skin Aging

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Solution Overview

Problem

Current skin care solutions fail to effectively prevent skin aging and wrinkle formation, particularly due to external stressors like UV light, as they do not adequately promote collagen and elastin synthesis or regeneration.

Innovation Solution

A functional composition combining an exosome-rich conditioned medium from immortalized stem cells and botulinum toxin, which promotes collagen and elastin synthesis, reduces wrinkles, and stimulates cell regeneration.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional skin care solutions are used, then basic skin protection is maintained, but they fail to effectively prevent skin aging and wrinkle formation

Engineering Contradiction:
Improveskin aging prevention effectivenessVSAvoidcollagen and elastin synthesis promotion
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent combines exosome-rich conditioned medium from immortalized stem cells with botulinum toxin in a single functional composition. This merging of two distinct active ingredients enables synergistic effects where the exosomes promote collagen and elastin synthesis while botulinum toxin prevents wrinkle formation, collectively addressing skin aging prevention more effectively than either ingredient alone.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The functional composition serves as a composite biological material integrating multiple active components: exosomes containing growth factors and neurotrophic factors from immortalized stem cells, combined with botulinum toxin. This composite formulation delivers multiple functions simultaneously - promoting tissue regeneration, enhancing skin elasticity, and preventing wrinkle formation - thereby improving reliability in skin aging prevention.

Inventive Principle:
Principle #40Composite materials

2Strength

If external stressors like UV light are exposed to skin, then skin damage occurs, but rapid restoration to normal stratum corneum is not achieved

Engineering Contradiction:
Improvestratum corneum restoration speedVSAvoidexternal stressor damage
Core Design Contradiction:
StrengthVSObject-affected harmful factors

Solution Approach 1:

The exosome-rich conditioned medium contains pre-formed growth factors and neurotrophic factors that are ready to immediately stimulate keratinocyte proliferation and collagen synthesis upon application. This preliminary preparation of regenerative components enables rapid response to skin damage, accelerating the restoration of the stratum corneum faster than natural healing processes alone.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Exosomes serve as intermediary carriers delivering growth factors and neurotrophic factors from immortalized stem cells to damaged skin tissues. These exosomes mediate the communication between the applied composition and the skin's regenerative machinery, facilitating coordinated tissue repair and enhancing the speed of stratum corneum restoration in response to UV damage.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Productivity

If skin regeneration is promoted through cellular interactions and growth factors, then wound healing is enhanced, but the complexity of cellular interactions is increased

Engineering Contradiction:
Improvewound healing efficiencyVSAvoidcellular interaction complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent extracts and isolates the essential regenerative components (growth factors and neurotrophic factors) from complex biological sources and concentrates them within exosomes. This extraction process simplifies the active ingredients to a defined set of proven effective molecules, making the composition more manageable and easier to control while maintaining high wound healing efficiency.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The composition utilizes parameter changes in the exosome structure and cargo composition to optimize delivery and activity. By controlling exosome size, surface markers, and enclosed factor concentrations, the system achieves enhanced cellular interactions and wound healing without requiring complex formulation adjustments, thereby improving productivity while managing complexity.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP4104842A1Functional composition comprising exosome-rich conditioned medium of immortalized stem cells and botulinum toxin
Publication Date: 2022.12.21 CHUNGBUK NATIONAL UNIVERSITY IND -ACADEMICCOOPERATION FOUNDATION
  • EP4104842A1 patent drawingFigure 1
  • EP4104842A1 patent drawingFigure 2
  • EP4104842A1 patent drawingFigure 3

AI summary

Disclosed herein is a functional composition comprising an exosome-rich conditioned medium of immortalized stem cells and botulinum toxin. Functioning to prevent the degradation of collagen and elastin, promote the synthesis of collagen and elastin, reduce wrinkles, and stimulate cell regeneration, the functional composition according to the present disclosure can find advantageous applications as a functional material for wrinkle reduction, skin regeneration, improvement in skin elasticity, and prevention of skin aging and wrinkle generation or in use for treating a wound.