Expression System Combining T and B Cell Immunity
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Solution Overview
Problem
Current expression systems for inducing immune responses against pathogens, such as viruses, often fail to elicit a strong enough protective immune response, particularly against viruses like RSV and influenza, which are challenging due to their efficient gene expression strategies and lack of effective vaccines.
Innovation Solution
Development of an expression system comprising polynucleotides encoding proteins or peptides that induce both T cell and anti-pathogenic B cell responses, including a polyprotein approach with a multibasic HA0 cleavage site for expressing influenza hemagglutinin, to enhance immune response effectiveness.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If current expression systems are used to induce immune responses, then the systems are simple and well-characterized, but the immune response strength is insufficient to provide full protection against infections
Solution Approach 1:
The patent combines multiple polynucleotides encoding different immunogenic proteins or peptides into a single expression system. Specifically, it merges polynucleotides encoding T cell epitopes with polynucleotides encoding B cell epitopes, creating a unified system that elicits both T cell and B cell responses, thereby achieving stronger protective immunity without requiring multiple separate administrations
Solution Approach 2:
The expression system uses composite immunogenic constructs that combine multiple protein components. The system includes composite polynucleotide sequences that encode multiple immunogenic proteins or peptides from the same or different pathogens, creating a composite antigenic material that stimulates broader and stronger immune responses compared to single-component systems
2Reliability
If expression systems encode multiple immunogenic proteins to strengthen immune response, then the protective effect improves, but the system complexity and manufacturing difficulty increase
Solution Approach 1:
The patent segments the complex immunogenic information into distinct modular polynucleotide components, each encoding a specific immunogenic protein or peptide. These modular segments can be independently designed, characterized, and manufactured, then assembled into the complete expression system. This segmentation facilitates standardized manufacturing processes and quality control while maintaining the complexity of multi-protein encoding
Solution Approach 2:
The expression system is designed with universal features that enable it to encode multiple immunogenic proteins from the same or different pathogens using common regulatory elements and structural frameworks. The system uses universal promoter sequences, ribosome binding sites, and termination signals that work across different protein编码 sequences, simplifying the manufacturing process by allowing standardized production protocols to be applied to various pathogen targets
Data Source
AI summary
The invention relates to an expression system comprising polynucleotides encoding proteins, wherein the expression system comprises a first polynucleotide encoding at least one protein, peptide or variant thereof, which induces a T cell response, and a second polynucleotide encoding at least one protein, peptide or variant thereof, which induces an anti-pathogenic B cell response. The invention further relates to protein mixtures encoded by the expression system and cells comprising the expression system or the protein mixture and pharmaceutical compositions comprising the expression system or the protein mixture.