Extracellular Vesicle Cryopreservation with Vinylpyrrolidone Polymer
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Solution Overview
Problem
Extracellular vesicles are susceptible to freezing and repeated freezing and thawing operations, which can reduce their number and affect their physiological activity.
Innovation Solution
Stabilize extracellular vesicles by cryopreserving them in the presence of a polymer containing a monomer unit derived from vinylpyrrolidone, such as a copolymer with vinyl acetate, using specific molecular weight and viscosity characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If cryopreservation is performed on extracellular vesicles, then the extracellular vesicles can be stored for long periods, but the number of extracellular vesicle particles is reduced due to freezing damage
Solution Approach 1:
The patent introduces a polymer with specific properties (containing monomer units derived from vinylpyrrolidone, with Fikentscher K value of 5-50 and weight-average molecular weight of 3,000-250,000) as an intermediary substance during cryopreservation. This polymer acts as a protective mediator that reduces freezing damage to extracellular vesicles, allowing long-term storage while maintaining particle quantity. The polymer is used at a concentration of 0.001-5% (w/v) in the storage solution.
Solution Approach 2:
The patent applies parameter changes by carefully controlling the molecular weight and viscosity characteristics of the polymer used in cryopreservation. By specifying the Fikentscher K value (5-50) and weight-average molecular weight (3,000-250,000), the invention optimizes the polymer's protective effect during freezing and thawing, thereby preserving both storage duration and particle quantity.
2Adaptability or versatility
If repeated freezing and thawing operations are performed on extracellular vesicles, then the extracellular vesicles can be used multiple times, but the number of extracellular vesicle particles is reduced
Solution Approach 1:
The polymer with specific properties serves as a protective intermediary during repeated freezing and thawing cycles. This mediator substance reduces mechanical and osmotic stress on extracellular vesicles during phase transitions, enabling multiple uses while maintaining particle quantity and functional integrity.
Solution Approach 2:
The patent applies beforehand cushioning by pre-treating extracellular vesicles with the protective polymer before subjecting them to freezing and thawing operations. This prior protection creates a cushioning effect that absorbs freezing damage, allowing the vesicles to withstand repeated thermal cycling without significant particle loss.
3Ease of manufacture
If cryopreservation is performed without protective agents, then the storage process is simple, but the quality of extracellular vesicles deteriorates
Solution Approach 1:
The patent maintains relative simplicity by using a polymer with well-defined parameter ranges (Fikentscher K value of 5-50, weight-average molecular weight of 3,000-250,000) rather than complex formulations. This parameter-based approach ensures consistent quality improvement while keeping the storage process straightforward and easily implementable.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Maintains the number and activity of extracellular vesicles during cryopreservation, reducing adverse effects from freezing and thawing.
Implementation Method 1
it is necessary to store the extracellular vesicle without deteriorating the quality thereof. For this reason, cryopreservation is general as the storage of the extracellular vesicle
Implementation Method 2
trehalose prevents aggregation of exosomes and cryodamage
Data Source
AI summary
An object of the present invention is to provide a means capable of stably storing an extracellular vesicle. The present invention relates to a storage stabilizer for an extracellular vesicle, which contains a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate, or a polymer having a monomer unit derived from vinylpyrrolidone such as polyvinylpyrrolidone and relates to a storage stabilization method for an extracellular vesicle, which includes cryopreserving an extracellular vesicle in the presence of the polymer.