Extracellular Vesicle Recovery Using Polymer-Mediated Separation
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Solution Overview
Problem
Existing methods for recovering extracellular vesicles suffer from low efficiency and uneven product quality due to unspecific concentration, particularly in complex biological samples like blood and urine.
Innovation Solution
A method involving the use of a polymer with specific viscosity properties, such as cellulose derivatives or polyvinyl derivatives, to separate extracellular vesicles from samples, optionally combined with chelating agents and membrane-binding materials, enhances recovery efficiency and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If immunoprecipitation method using antibody against extracellular vesicle marker is used, then extracellular vesicles can be recovered, but recovery efficiency is low and product quality is uneven due to unspecific concentration
Solution Approach 1:
The patent introduces a polymer as an intermediary substance that mediates the separation process. The polymer interacts with extracellular vesicles through specific mechanisms (such as hydrophobic interactions or charge interactions) to enable efficient and specific separation, replacing the antibody-based immunoprecipitation method that suffers from low efficiency and uneven results
Solution Approach 2:
The patent changes the separation parameter from biological recognition (antibody-antigen binding) to physical-chemical properties (polymer-vesicle interactions based on hydrophobicity, charge, or size). By adjusting polymer concentration, molecular weight, and chemical properties, the method achieves consistent and high-efficiency recovery with uniform product quality
2Productivity
If ultracentrifugal method is used to recover extracellular vesicles, then separation can be achieved, but recovery efficiency remains insufficient and product quality is inconsistent
Solution Approach 1:
The polymer serves as a mediator that enhances the separation process. Instead of relying solely on centrifugal force to separate vesicles based on density, the polymer creates additional interaction mechanisms that improve separation efficiency and consistency, leading to higher recovery rates and more uniform product quality
Solution Approach 2:
The patent combines the ultracentrifugal separation method with polymer-based interaction mechanisms to create a composite separation system. This combination leverages both mechanical force (centrifugation) and chemical/physical interactions (polymer-vesicle binding) to achieve superior separation performance compared to either method alone
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high-efficiency and high-purity recovery of extracellular vesicles, particularly from complex biological samples, enabling precise analysis of their components.
Implementation Method 1
the polymer has a value of 1.5 mPa·s or more as a viscosity in 1 to 20 wt % aqueous solution at 20 to 30° C.
Data Source
AI summary
The present invention provides a method of recovering an extracellular vesicle from an extracellular vesicle-containing sample. More specifically, the present invention provides a method of recovering the extracellular vesicle that includes separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.