Extracellular Vesicle Stabilization with Saccharides and Chelators
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Solution Overview
Problem
Existing methods fail to effectively stabilize extracellular vesicles for preservation and application in diagnosis and drug discovery.
Innovation Solution
Stabilization of extracellular vesicles is achieved by mixing a sample containing them with a saccharide and a chelating agent, followed by freezing, preferably through freeze-drying.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If extracellular vesicles are treated with conventional stabilization methods, then some preservation is achieved, but the yield and stability are insufficient for diagnostic and drug discovery applications
Solution Approach 1:
The patent applies parameter changes by optimizing the concentration of EDTA (chelating agent) to a specific range of 1-100 μM and controlling the pH value within 7.0-9.0. These parameter optimizations enable simultaneous improvement of both extracellular vesicle yield and stability, resolving the contradiction between productivity and reliability in conventional methods
Solution Approach 2:
The patent employs a composite stabilization system combining EDTA (chelating agent) with specific saccharides (such as sucrose, trehalose, or mannitol) at defined concentrations. This composite approach creates a synergistic effect that enhances both the yield and stability of extracellular vesicles, overcoming the limitations of single-agent methods
2Productivity
If high concentration of EDTA is used to improve yield, then extracellular vesicle yield increases, but the stability and integrity of extracellular vesicles deteriorates
Solution Approach 1:
The patent resolves this contradiction by precisely controlling the EDTA concentration parameter within the optimal range of 1-100 μM rather than using high concentrations. This parameter optimization allows achieving sufficient yield while maintaining vesicle stability and integrity, preventing the deterioration that occurs at elevated EDTA levels
3Duration of action of stationary object
If freezing and thawing is performed to stabilize extracellular vesicles, then preservability improves, but the membrane structure and function may be damaged
Solution Approach 1:
The patent applies preliminary action by pre-treating extracellular vesicles with EDTA and saccharides before freezing and thawing procedures. This preliminary stabilization protects the membrane structure during subsequent freezing-thawing cycles, enabling long-term preservation while maintaining structural integrity and functional properties
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enhances the preservability of extracellular vesicles, making them suitable for diagnostic and drug discovery applications.
Implementation Method 1
mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent
Implementation Method 2
freezing a mixture of the extracellular vesicle-containing sample and the saccharide
Data Source
AI summary
The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.