Complement Factor H Detection via Light Scattering
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Solution Overview
Problem
Current methods for detecting complement factor H in human circulation, such as ELISA and RIA, are inefficient due to long detection times, complex operation steps, and inability to quickly and accurately detect large batches, and immunoturbidimetry methods fail to accurately detect complement factor H.
Innovation Solution
A method utilizing low-molecular weight polyethylene glycol (PEG) and principles of immunoturbidimetry and immunonephelometry to detect complement factor H in plasma, serum, or urine, allowing for rapid and accurate detection of multiple samples with simple operation and storage conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ELISA or RIA methods are used to detect complement factor H, then detection accuracy can be maintained, but detection time becomes long and operation steps become complex
Solution Approach 1:
The patent replaces the complex mechanical/chemical procedures of ELISA and RIA with an optical detection method based on light scattering. The immunocomplex formation is detected through changes in light scattering properties, eliminating the need for washing, substrate addition, and colorimetric development steps, thereby reducing detection time while maintaining accuracy
Solution Approach 2:
The patent changes the detection parameter from enzymatic colorimetric signals (in ELISA/RIA) to light scattering intensity. By measuring the scattering of light at specific angles and wavelengths, the method achieves rapid quantification of complement factor H concentrations without the time-consuming steps required by traditional methods
2Measurement precision
If ELISA or RIA methods are used to detect complement factor H, then detection accuracy can be maintained, but operation complexity increases
Solution Approach 1:
The patent replaces multiple manual操作步骤 (washing, incubation, substrate addition) with a single optical measurement step. The light scattering detector automatically measures the immunocomplex formation in real-time, eliminating the need for complex manual operations while preserving detection accuracy
Solution Approach 2:
The detection system performs automatic measurement of light scattering properties without requiring manual intervention for washing, reagent addition, or signal development. The system self-regulates the detection process, making operation simple while maintaining the precision of antibody-antigen interaction detection
3Productivity
If traditional immunoturbidimetry methods are used, then detection speed can be improved, but detection accuracy for complement factor H fails
Solution Approach 1:
The patent applies local quality by using latex particles with specific surface properties that selectively enhance binding to complement factor H-antibody immunocomplexes. The modified latex particles provide localized binding sites that improve both the speed and accuracy of detection specifically for complement factor H, distinguishing it from traditional immunoturbidimetry
4Productivity
If batch detection is required, then productivity can be improved, but detection accuracy and speed decrease with current methods
Solution Approach 1:
The patent creates a universal detection system using standardized latex particle suspensions and buffer solutions that can process multiple samples simultaneously with consistent results. The method maintains the same detection accuracy for single samples and batch samples, enabling high-throughput screening without compromising precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high sensitivity and efficiency in detecting complement factor H concentrations within 5-10 minutes, with a wide detection range of 4.86-2000 mg/L, and can handle samples stored at varying temperatures without affecting results, making it suitable for clinical applications.
Implementation Method 1
the present invention utilizes the low-molecular weight polyethylene glycol, adopts the principles of immunity transmission turbidimetry (immunoturbidimetry) and scattered transmission turbidimetry (immunonephelometry) to provide a method that can quickly detect the level of complement factor H
Implementation Method 2
detecting to obtain a second absorbance value A2 of the sample to be detected; adding the reagent I consisting of the Tris buffer and the PEG200 solution to a standard sample
Data Source
AI summary
A method comprises adding reagent I to a sample to be detected, incubating to obtain a first detection sample, and detecting to obtain a first absorbance value A1 of the sample to be detected; adding reagent II to the first detection sample, incubating to obtain a second detection sample, and detecting to obtain a second absorbance value A2 of the sample to be detected; adding reagent I to a standard sample, incubating to obtain a first detection standard, and detecting to obtain a first absorbance value B1 of the standard; adding reagent II to the first detection standard, incubating to obtain a second detection standard, and detecting to obtain a second absorbance value B2 of the standard; and using A1 and A2, and B1 and B2 to obtain a concentration level of complement factor H in the sample to be detected.