FAGE Biofuel Composition via Enzymatic Transesterification of FAME

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

The production of biodiesel results in the generation of crude glycerin as a coproduct, which lacks widespread commercial applications due to its impurity, and existing methods do not effectively convert fatty acid methyl esters (FAME) into fatty acid glycerol formal esters (FAGE) using enzymatic transesterification.

Innovation Solution

An enzymatic transesterification process using lipolytic enzymes, particularly Thermomyces lanuginosus lipase, Candida antarctica lipase B, and Proteus sp. K107 lipase, converts FAME into FAGE in the presence of glycerol formal, producing a biofuel composition suitable for diesel blends.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical catalysts (Titanium catalyst, acid catalysts) are used for transesterification to produce FAGE, then the reaction can proceed, but the process is not enzymatically driven and lacks the efficiency and cleanliness of enzymatic methods

Engineering Contradiction:
Improveenzymatic catalysisVSAvoidprocess complexity
Core Design Contradiction:
ReliabilityVSEase of manufacture

Solution Approach 1:

The patent replaces chemical catalysts (Titanium catalyst, acid catalysts) with enzymatic catalysts (lipolytic enzymes) for the transesterification reaction. This substitution eliminates the need for harsh chemical conditions, achieves higher specificity and efficiency, and enables easier product separation due to the biodegradable nature of enzymes, thereby resolving the contradiction between reliability of catalysis and ease of manufacture.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Ease of manufacture

If crude glycerin is used directly in FAME production, then the process is simple, but the impure composite has limited commercial applications due to lack of purification

Engineering Contradiction:
Improveprocess simplicityVSAvoidcommercial applications
Core Design Contradiction:
Ease of manufactureVSAdaptability or versatility

Solution Approach 1:

The patent converts crude glycerin, which is an impure byproduct with limited commercial value, into FAGE through enzymatic transesterification. This transformation turns the harmful/limiting impurity into a valuable biofuel product, expanding commercial applications while maintaining process simplicity by using the crude glycerin directly without extensive purification steps.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent changes the chemical parameters of crude glycerin by transforming it into FAGE through enzymatic catalysis. This parameter change (from impure glycerol to ester compound) enables the substance to move from having limited commercial applications to becoming a viable biofuel product, resolving the contradiction between ease of manufacture and adaptability.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If conventional transesterification methods are used to produce FAME, then biodiesel production is achieved, but crude glycerin is generated as an unwanted coproduct

Engineering Contradiction:
Improvebiodiesel productionVSAvoidcrude glycerin
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The patent converts the unwanted coproduct crude glycerin into a valuable biofuel component FAGE through enzymatic transesterification. This transforms the loss of substance (crude glycerin) into a beneficial product, thereby resolving the contradiction between productivity in biodiesel production and the loss of valuable byproducts.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent makes the enzymatic system multi-functional by using lipolytic enzymes to perform both the desired FAME production and the simultaneous conversion of crude glycerin into FAGE. This multi-functionality eliminates waste and maximizes product yield, resolving the contradiction between productivity and substance loss.

Inventive Principle:
Principle #6Universality (Multi-functionality)

4Ease of repair

If immobilized enzymes are used for transesterification, then enzyme recovery is facilitated, but the device complexity increases

Engineering Contradiction:
Improveenzyme recoveryVSAvoidimmobilization system
Core Design Contradiction:
Ease of repairVSDevice complexity

Solution Approach 1:

The patent implements enzyme recovery through immobilization techniques, allowing the enzymes to be separated and reused after the reaction. This facilitates ease of repair and resource utilization, resolving the contradiction between ease of enzyme recovery and device complexity by using established immobilization methods that balance recoverability with system simplicity.

Inventive Principle:
Principle #34Discarding and recovering

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process achieves high yields of FAGE with short reaction times and facilitates enzyme recovery, resulting in a sustainable biofuel composition compatible with diesel standards, effectively valorizing crude glycerin.

Implementation Method 1

The process herein described, entails the conversion of fatty acid methyl esters (FAME) into fatty acid glycerol formal esters (FAGE) via enzymatic transesterification

Methodology Applied
Scientific EffectEnzymatic catalysis: Enzyme

Implementation Method 2

conversion of fatty acid methyl esters (FAME) into fatty acid glycerol formal esters (FAGE) via enzymatic transesterification

Methodology Applied
Scientific EffectTransesterification: Chemical Bonding

Data Source

PatentEP4610335A1Method for preparing a biofuel composition
Publication Date: 2025.09.03 SELABTEC SCI SLU
  • EP4610335A1 patent drawingFigure 1
  • EP4610335A1 patent drawingFigure 2
  • EP4610335A1 patent drawingFigure 3

AI summary

The present invention relates to a process for producing a FAGE-based biofuel composition, the process being characterized by an enzymatic transesterification of fatty acid alkyl esters with glycerol formal. The invention is also directed to the biofuel composition obtained by said process as well as to the uses thereof.