SNP-Based Phytoplasma Diagnostic Assay for Grapevine FD Detection
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Solution Overview
Problem
Current methods for detecting Flavescence dorée (FD) phytoplasma in grapevines are unreliable due to low and fluctuating pathogen titers, irregular distribution, and the need for lengthy and costly diagnostic processes, which complicates distinguishing FD from other phytoplasmas, leading to inaccurate diagnoses and unnecessary eradication of healthy rootstocks.
Innovation Solution
A new nucleotide sequence specific to the rpl14 gene of FD phytoplasmas is identified, allowing for the development of a diagnostic assay that uses single nucleotide polymorphisms (SNPs) at positions 108 and 171 to specifically detect FD phytoplasmas, enabling rapid and accurate identification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional diagnostic methods are used to detect Flavescence dorée phytoplasma, then detection can be performed, but the diagnostic process is lengthy and costly, and accuracy is low due to low and fluctuating pathogen titers
Solution Approach 1:
The patent changes the detection parameter from general phytoplasma detection to specific SNP detection at positions 108 and 171 of the rpl14 gene. This parameter change enables specific identification of FD phytoplasma sequences, improving diagnostic accuracy while reducing the time required for diagnosis through targeted molecular detection methods.
2Measurement precision
If conventional diagnostic methods are used, then detection can be performed, but the cost of diagnosis is high
Solution Approach 1:
The patent extracts and utilizes only the specific SNP regions at positions 108 and 171 of the rpl14 gene for diagnosis, rather than analyzing the entire genome or using broad-spectrum detection methods. This extraction of critical diagnostic information reduces reagent costs, simplifies the diagnostic process, and lowers overall diagnostic expenses while maintaining high accuracy.
3Reliability
If conventional diagnostic methods are used, then detection can be performed, but false positives occur leading to unnecessary eradication of healthy rootstocks
Solution Approach 1:
The patent applies local quality by focusing detection on specific SNP positions (108 and 171) within the rpl14 gene that are unique to FD phytoplasma. This localized specific detection approach distinguishes FD phytoplasma from other phytoplasma species, eliminating false positives and preventing unnecessary eradication of healthy rootstocks while maintaining high diagnostic reliability.
Data Source
AI summary
The present invention relates to a new genomic sequence specific for the phytoplasma causing Flavescence doree (FD) in vines, to a method for the diagnosis of FD in vines and parts thereof including rootstocks, and to a kit for the detection of the phytoplasma agents of FD from plant samples.