Fe(II) Ion Detection Agent Using Chelating Agent Composite
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Solution Overview
Problem
Current fluorescence probes for detecting Fe(II) ions have low detection sensitivity and selectivity, making it difficult to acutely detect changes in Fe(II) ion concentrations, which is a limitation for research and clinical applications related to diseases associated with abnormal iron levels.
Innovation Solution
A mixture of an Fe(II) ion detection agent comprising a compound represented by formula (I) (fluorescence probe) combined with a compound having at least three coordinating positions, such as nitrilotriacetic acid (NTA) or ethylenediaminetetraacetic acid (EDTA), which enhances the sensitivity and response rate of the fluorescent detection of Fe(II) ions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a traditional fluorescence probe is used to detect Fe(II) ions, then the detection selectivity is improved, but the detection sensitivity and response rate remain low
Solution Approach 1:
The patent combines a fluorescence probe with a chelating agent to create a composite detection system. The chelating agent forms stable complexes with Fe(II) ions, enhancing both the sensitivity and selectivity of detection while maintaining the fluorescence response characteristics of the probe.
Solution Approach 2:
The patent modifies the chemical environment by introducing chelating agents that alter the coordination chemistry of Fe(II) ions. This changes the detection parameters by enhancing the binding affinity and stability of the probe-ion complex, thereby improving detection sensitivity without compromising selectivity.
2Reliability
If a fluorescence probe is used to detect Fe(II) ions, then the detection capability is achieved, but the measurement time is excessively long (one hour)
Solution Approach 1:
The chelating agent pre-complexes with Fe(II) ions before the fluorescence probe binds, accelerating the overall detection process. This preliminary action reduces the time required for the probe to reach its binding equilibrium with the target ions.
Solution Approach 2:
The chelating agent acts as an intermediary that facilitates faster binding between the fluorescence probe and Fe(II) ions. By mediating the interaction through pre-formed complexes, it significantly reduces the measurement time while maintaining detection reliability.
3Measurement precision
If existing Fe(II) detection probes are used, then Fe(II) ion detection is possible, but the response rate is slow and cannot acutely detect concentration changes
Solution Approach 1:
The composite system of fluorescence probe and chelating agent creates multiple binding sites and enhances the overall affinity for Fe(II) ions, enabling faster equilibrium establishment and higher response rates while maintaining accurate detection of concentration changes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Fe(II) ion detection agent allows for rapid and highly sensitive detection of Fe(II) ions, significantly reducing the time required for measurement from one hour to five to ten minutes, enabling quick and quantitative detection in both test tubes and cells, thus facilitating research and clinical applications.
Implementation Method 1
the N-oxide moiety reacts with an Fe(II) ion to thereby undergo deoxygenation, thus increasing fluorescence
Implementation Method 2
the N-oxide moiety reacts with an Fe(II) ion to thereby undergo deoxygenation
Implementation Method 3
a compound having at least three coordinating positions, such as nitrilotriacetic acid (NTA) or ethylenediaminetetraacetic acid (EDTA)
Data Source
Figure 1~2(f)
Figure 3(a)~3(h)
Figure 4(a)~4(e)
AI summary
The present invention provides an Fe(II) ion detection agent capable of fluorescently detecting Fe(II) ions quickly with high sensitivity, and a method for detecting Fe(II) ions using the agent. The invention relates to an Fe(II) ion detection agent comprising a compound represented by formula (I) (fluorescence probe): wherein R1 and R2 represent lower alkyl and the like; R3 and R4 represent hydrogen and the like; R5 represents hydrogen, hydroxyl, or a group represented by formula (A) : -NR54R52 wherein R51 and R52 represent lower alkyl and the like; R6 and R7 represent hydrogen and the like; ring A represents an aromatic ring and the like; V represents O or SiR10R11 wherein R10 and R11 are the same or different and represent hydrogen or lower alkyl; W represents CH2, CO, and the like; Z represents O, and the like; m and n are the same or different and represent 0 or 1, in combination with a compound having at least three coordinating positions, and the invention also relates to a method for detecting Fe(II) ions using the agent.