Fermented Crude Extract ACE Inhibiting Activity
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Solution Overview
Problem
Current methods for regulating blood pressure in hypertension patients using chemical synthesized drugs often come with side effects, and there is a need for more materials that can enhance the angiotensin converting enzyme (ACE) inhibiting activity in fermented products.
Innovation Solution
A method involving the addition of dried and milled residue materials such as sunflower seeds, wheat, and sesame residue into a culture medium for lactic acid bacteria fermentation, followed by specific hydrolysis and fermentation steps, to produce a fermented crude extract with ACE inhibiting activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical synthesized drugs are used to inhibit ACE activity, then blood pressure control is effective, but side effects such as dizziness, vomiting, fatigue, dry cough, high potassium, and kidney failure occur
Solution Approach 1:
The patent changes the chemical nature of the ACE inhibiting agent from synthetic drugs to naturally derived fermented crude extract. By altering the source and composition parameters (using lactic acid bacteria fermentation of residue materials), the therapeutic effect is maintained while eliminating the harmful side effects associated with synthetic drugs
Solution Approach 2:
The patent uses inexpensive residue materials (agricultural by-products) as the base for fermentation instead of expensive synthetic drug production. These residue materials serve as a sustainable, low-cost substrate that can be readily disposed of or composted after extraction, replacing costly pharmaceutical manufacturing
2Reliability
If natural materials are added to culture medium for LAB fermentation, then ACE inhibiting activity is enhanced, but the choices for materials are limited
Solution Approach 1:
The patent establishes a universal fermentation system using lactic acid bacteria that can process multiple types of residue materials (vegetable, fruit, grain, and other agricultural by-products). This multi-functional approach allows the same fermentation methodology to be applied across diverse material types, greatly expanding the versatility of material selection while maintaining enhanced ACE inhibiting activity
Solution Approach 2:
The patent employs composite materials by combining residue materials with culture medium components for LAB fermentation. This composite approach creates a synergistic system where the residue material provides substrate diversity and the culture medium provides optimal fermentation conditions, thereby expanding material options while ensuring consistent ACE inhibiting effects
3Reliability
If residue materials are used for fermentation, then health-care products with ACE inhibiting activity are produced, but the production process requires multiple steps including drying, milling, hydrolysis, and fermentation
Solution Approach 1:
The patent applies preliminary action by pre-processing the residue materials through drying and milling before fermentation. These preliminary steps prepare the materials in an optimal state for subsequent enzymatic hydrolysis and fermentation, ensuring consistent quality and ACE inhibiting activity in the final product while streamlining the overall production process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively produces a fermented crude extract with ACE inhibiting activity, potentially reducing blood pressure without the side effects of chemical drugs, and allows for the use of various residue materials to achieve better health-care products.
Implementation Method 1
0.1 vol % α-amylase is added in the mixture solution to perform hydrolysis at 95° C. for 1 hour
Implementation Method 2
0.1 vol % glucoamylase is added in the mixture solution to perform hydrolysis at 65° C. for 4 hours
Implementation Method 3
1% (w/w) of a lactic acid bacterium is added in a culture medium containing the mixture solution to perform fermentation for 24 hours
Implementation Method 4
The culture medium is centrifuged to take the supernatant thereof
Implementation Method 5
the supernatant is boiled for 20 minutes
Data Source
AI summary
This invention is related to a method of preparing a fermented crude extract having angiotensin converting enzyme inhibiting activity. The method comprises the following steps. A material is dried, milled, and then mixed with water in a weight ratio of 1:11 to form a mixture solution. 0.1 vol % α-amylase is used to perform hydrolysis at 95° C. for 1 hour. 0.1 vol % glucoamylase is used to perform hydrolysis at 65° C. for 4 hours. A lactic acid bacterium is added in a culture medium containing the mixture solution to perform fermentation for 24 hours. The culture medium is centrifuged to take the supernatant thereof, and the supernatant is boiled for 20 minutes. The supernatant is filtered to obtain a fermented crude extract having angiotensin converting enzyme inhibiting activity.