Ferritin Affinity Purification With High-Affinity Binding Proteins
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Solution Overview
Problem
Current methods for purifying ferritin are inefficient and lack precision, hindering its effective use in medical applications and drug delivery.
Innovation Solution
Development of novel binding proteins with high affinity for ferritin, allowing precise purification via affinity chromatography, including fusion proteins with specific binding sites for solid support attachment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional purification methods are used, then ferritin can be obtained, but the purification is inefficient and lacks precision
Solution Approach 1:
The patent introduces a binding protein as an intermediary substance that specifically binds to ferritin. This binding protein acts as a mediator between the ferritin to be purified and the solid support, enabling selective capture and precise purification of ferritin while maintaining high efficiency through the specific binding interaction.
Solution Approach 2:
The patent replaces conventional mechanical or chemical purification methods with a biological binding-based approach. Instead of using traditional chromatography or precipitation methods, the invention uses specific protein-protein binding interactions between the binding protein and ferritin to achieve purification, providing both precision and efficiency.
2Manufacturing precision
If high affinity binding proteins are used, then precise purification is achieved, but the binding affinity must be optimized for elution
Solution Approach 1:
The patent utilizes changes in environmental parameters, specifically pH, to modulate the binding affinity between the binding protein and ferritin. The binding protein is designed to bind ferritin tightly at neutral pH for capture, but the binding affinity decreases at acidic pH, enabling efficient elution of purified ferritin without requiring complex optimization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient and precise purification of ferritin, facilitating its use in medical applications and drug delivery systems.
Implementation Method 1
the binding protein has a binding affinity of less than 100 nM for ferritin
Implementation Method 2
contacting said affinity separation matrix with the liquid under conditions that permit binding of the at least one binding protein for ferritin
Data Source
AI summary
The present invention relates to novel proteins that specifically bind to the ferritin. The novel proteins of the present invention are advanced and powerful tools because they allow precise purification methods of ferritin, for example via affinity chromatography. Further, the binding protein for ferritin is useful for methods to analyze the presence of ferritin.