Fetal Hemoglobin Induction Compounds for Sickle Cell Disease

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Solution Overview

Problem

Current therapies for sickle cell disease and beta-thalassemia, such as regular transfusions and bone marrow transplantation, come with significant side effects and limitations, while pharmacological induction of fetal hemoglobin (HbF) using agents like hydroxyurea has limited efficacy and side effects, necessitating the development of alternative compounds to induce endogenous embryonic/fetal globin expression.

Innovation Solution

Development of compounds, such as those of Formula (I), that induce the expression of endogenous embryonic/fetal globin chains, particularly γ globin, to treat anemia and related symptoms in sickle cell disease and beta-thalassemia, offering a potential alternative to existing treatments.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If regular transfusion is used to replenish functional HbA, then survival requirement is met, but iron overload causing tissue damage and organ dysfunction occurs

Engineering Contradiction:
ImprovesurvivalVSAvoidiron overload
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of excess alpha-globin chains (which cause toxicity and anemia) into a beneficial outcome by inducing fetal hemoglobin production. The gamma-globin chains from HbF pair with excess alpha-globin chains to form functional tetramers, transforming the toxic excess into useful functional hemoglobin and avoiding the need for iron-chelation therapy

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent introduces fetal hemoglobin (HbF) as an intermediary substance that mediates between the excess alpha-globin chains and the need for functional hemoglobin. The gamma-globin chains act as intermediaries that can pair with alpha-globin chains, creating a bridge that resolves the incompatibility between excess alpha-chains and the requirement for balanced globin chain assembly

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If bone marrow transplantation is performed to treat severe SCD and beta-thalassemia, then severe anemia can be cured, but identification of matched donor is limited and immune response risk increases

Engineering Contradiction:
Improvecure of severe anemiaVSAvoiddonor matching complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent enables the patient's own cells to produce the therapeutic agent (fetal hemoglobin) through pharmacological induction. The endogenous gamma-globin genes are reactivated in the patient's own erythroid cells, eliminating the need for external donor cells and avoiding all associated complexities of donor matching and immune rejection

Inventive Principle:
Principle #25Self-service

3Reliability

If pharmacological induction of HbF using hydroxyurea is applied, then some efficacy is achieved, but limited efficacy and side effects remain

Engineering Contradiction:
ImproveHbF induction efficacyVSAvoidside effects
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent employs a series of compounds with progressively optimized molecular parameters to induce gamma-globin expression. The compounds differ in their chemical structures (substituted benzylidene guanidines with various aromatic groups), and the invention selects compounds based on their ability to achieve higher HbF induction efficacy with improved safety profiles compared to hydroxyurea

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentEP3947382B1Compounds for uses in pharmacological induction of hbf for treatment of sickle cell disease and beta-thalassemia
Publication Date: 2025.09.24 ACAD SINICA
  • EP3947382B1 patent drawingFigure 1A
  • EP3947382B1 patent drawingFigure 1B
  • EP3947382B1 patent drawingFigure 2~3

AI summary

Provided herein are compounds of Formula (I). The compounds described herein are useful in treating a disease associated with the expression of endogenous embryonic/fetal globin (e.g., γ globin) in erythrocytes (e.g., treating β -thalassemia and/or sickle cell disease through at least the induction of the globin gene expression in erythrocytes), and/or anemia (β-thalassemia and/or sickle cell anemia). The compounds described herein are useful in treating, delaying, and/or preventing the adverse effects of β-thalassemia and/or sickle cell disease, inducing γ globin production, and/or inducing the expression of embryonic/fetal globin genes in a subject, cell, tissue, or biological sample. Also provided in the present disclosure are pharmaceutical compositions, kits, and methods of using the compounds for inducing γ globin production described herein and for treating any of the target diseases described herein.