Water-Insoluble Fiber Adsorbent for Bioparticle Purification
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Solution Overview
Problem
Current methods for purifying biological particles, such as viruses, are expensive and inefficient due to the high cost and small pore diameters of bead carriers used in traditional chromatography, leading to low recovery rates and high residual rates in the washing step.
Innovation Solution
A structure comprising a water-insoluble fiber with a low-molecular-weight antibody connected to it, which serves as an adsorbent for efficient purification of biological particles, offering a high recovery rate in the elution step and low residual rate in the washing step, while being cost-effective.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If bead carriers are used in traditional chromatography, then purification can be performed, but the cost becomes expensive and the pore diameters become small
Solution Approach 1:
The patent uses porous glass beads as the base material for the adsorbent carrier. These porous materials provide large pore diameters that enable efficient passage of biological particles while maintaining structural integrity and purification effectiveness, directly addressing the pore diameter limitation of traditional bead carriers
Solution Approach 2:
The patent employs a cost-effective adsorbent composition using porous glass beads combined with low-molecular-weight antibodies. This combination provides an inexpensive alternative to traditional expensive bead carriers while maintaining purification effectiveness, making the process economically viable for large-scale applications
2Reliability
If traditional chromatography methods are used, then biological particles can be purified, but the recovery rate becomes low and residual rate in washing step becomes high
Solution Approach 1:
The patent optimizes the adsorbent structure by combining porous glass beads with low-molecular-weight antibodies at specific concentrations. This parameter optimization enhances the binding efficiency and selectivity, resulting in improved recovery rates during elution and reduced residual rates during washing steps
Solution Approach 2:
The patent creates a composite adsorbent material by immobilizing low-molecular-weight antibodies onto porous glass bead surfaces. This composite structure combines the advantages of porous materials (large pore diameter, high surface area) with the specific binding capability of antibodies, achieving both high purification effectiveness and high productivity
3Strength
If bead carriers with small pore diameters are used, then structural integrity is maintained, but efficient recovery of biological particles is hindered
Solution Approach 1:
The patent specifically selects porous glass beads with controlled pore diameters that are optimized for biological particle passage. These porous materials maintain structural integrity while providing sufficient pore size to allow efficient recovery of viruses and other biological particles, directly resolving the contradiction between strength and productivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The described method provides an efficient and cost-effective means to purify biological particles with high recovery rates and low residual rates, addressing the limitations of traditional chromatography techniques.
Implementation Method 1
Affinity chromatography is a method of separating target substances and other impurities by using carriers obtained by immobilizing, to bead base materials, ligands such as antibodies or the like that specifically bind to target molecules.
Implementation Method 2
a structure including a water-insoluble fiber and a low-molecular-weight antibody connected to the water-insoluble fiber
Implementation Method 3
ligands such as antibodies or the like that specifically bind to target molecules
Data Source
AI summary
A structure includes a water-insoluble fiber, and a low-molecular-weight antibody connected to the water-insoluble fiber. A method of producing a structure includes connecting a water-insoluble fiber and a low-molecular-weight antibody to each other.