Fibrin Gel Sheet Composition for Uniform Cell Dispersion at Larger Sizes
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Solution Overview
Problem
Existing sheet-shaped structures for cell transplantation using biodegradable gels are insufficient in size for large mammals and have non-uniform cell distribution, which can reduce treatment efficiency and long-term engraftment.
Innovation Solution
Adjusting the amounts of fibrinogen and thrombin and incorporating biodegradable particles or gelators to control gelation, allowing for the formation of a fibrin gel sheet with uniform cell dispersion and larger size.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If a larger amount of fibrin gel is prepared to create a larger size sheet, then the sheet size increases, but the gelation occurs before the sheet can be sufficiently expanded, making it difficult to shape
Solution Approach 1:
The patent adjusts the fibrinogen concentration and thrombin concentration to control the gelation kinetics. By optimizing these parameters, the gelation occurs at an appropriate rate that allows sufficient time for sheet expansion and shaping while still achieving the desired large size. This parameter optimization resolves the contradiction between sheet size and shaping ease.
2Productivity
If continuous injection is used to shape a sheet of desired size, then the sheet can be formed efficiently, but cells cause precipitation and cell density becomes non-uniform
Solution Approach 1:
The patent introduces biodegradable particles into the fibrinogen solution containing cells. These particles serve as a composite component that interacts with the fibrin network during gelation to prevent cell precipitation. The composite structure of fibrin gel with embedded biodegradable particles maintains uniform cell distribution while allowing continuous injection for efficient sheet formation.
3Speed
If the gelation rate is fast, then the sheet structure is formed quickly, but cell distribution becomes non-uniform due to precipitation
Solution Approach 1:
The patent optimizes the thrombin concentration and fibrinogen concentration to achieve an appropriate gelation rate. By carefully controlling these parameters, the gelation proceeds at a speed that is fast enough to form the sheet structure efficiently but slow enough to prevent cell precipitation and maintain uniform cell dispersion throughout the gel matrix.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a fibrin gel sheet with a larger size and uniform cell distribution, enhancing treatment efficiency and long-term engraftment of transplanted cells.
Implementation Method 1
the amounts of fibrinogen and thrombin blended and/or a temperature at the time of gelation (solidification) is adjusted, whereby the gelation (solidification) rate of the fibrin gel to be formed can be adjusted
Implementation Method 2
biodegradable particles are added and suspended in a fibrinogen solution of cells suspended therein, or a biodegradable gelator is added and dissolved therein, whereby the cells are prevented from being precipitated in the fibrinogen solution
Data Source
AI summary
An object of the present invention is to provide a fibrin gel sheet for cell transplantation that has an unprecedented larger size while cells are uniformly dispersed and embedded, and a method for producing the same. The present invention provides a fibrin gel sheet for cell transplantation, wherein cells are uniformly dispersed and embedded in the fibrin gel sheet, and the fibrin gel sheet has a size of 2.25 cm2 or larger in terms of a surface area of one surface and has a thickness of 1 mm or smaller, and a method for producing the same.