Fibroblast Collagen III Composition for Skin Regeneration
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Solution Overview
Problem
Current methods for supplementing the extracellular matrix, such as using single polysaccharide components or inducing inflammation with polylactic acid microspheres, are inadequate as they either provide temporary mechanical support or cause side effects due to inflammatory stimulation.
Innovation Solution
A method for preparing a collagen type III composition from fibroblast extracellular matrix, involving the isolation and extraction of collagen type III from in vitro culture supernatant of human fibroblasts, and combining it with hyaluronic acid and an active polypeptide to enhance skin anti-aging effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If single polysaccharide components are used to supplement extracellular matrix, then mechanical support is provided, but the support is only temporary and lacks regenerative capability
Solution Approach 1:
The patent uses a composite material system consisting of fibroblast extracellular matrix combined with hyaluronic acid and polylactic acid microspheres. This composite approach provides both immediate mechanical support and sustained regenerative effects, resolving the contradiction between providing strength and maintaining duration of action.
2Quantity of substance
If polylactic acid microspheres are used to induce inflammation for extracellular matrix synthesis, then extracellular matrix production is stimulated, but strong side effects occur due to inflammatory stimulation
Solution Approach 1:
The patent introduces fibroblast extracellular matrix as an intermediary substance that mediates between the polylactic acid microspheres and the target tissue. This intermediary provides a biological framework that guides controlled extracellular matrix synthesis while reducing uncontrolled inflammatory responses and associated side effects.
3Reliability
If fibroblast extracellular matrix is isolated from in vitro culture supernatant, then a natural collagen source is obtained, but the isolation and extraction process is complex
Solution Approach 1:
The patent establishes a multi-functional isolation and extraction system that simultaneously achieves collagen type III purification, concentration, and validation. The method integrates multiple functions into a unified process, reducing operational complexity while maintaining high reliability of the natural collagen source.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The collagen type III composition effectively promotes fibroblast proliferation and extracellular matrix secretion, providing sustained mechanical support and inducing natural skin regeneration, thereby reducing skin wrinkles and improving skin health.
Implementation Method 1
a large amount of cell culture supernatant containing a large amount of human fibroblast extracellular matrix will be produced
Implementation Method 2
Some types of collagen triple helixes can be combined into an ordered polymer in which the collagen triple helixes are parallel to each other. This ordered polymer is called collagen fibril
Implementation Method 3
In tendon tissue, for example, fibroblasts can pull the collagen fibres to move directionally, so that they are distributed along the axis of tension in the tissue
Implementation Method 4
The common feature of non-collagen glycoproteins is that they can bind to both cells and other macromolecules in the extracellular matrix, thereby adhering cells to the extracellular matrix
Implementation Method 5
Proteoglycans and aminoglycans, also known as proteoglycans or mucopolysaccharides, are glycoproteins. Their sugar chains are mostly long-chain aminopolysaccharides
Data Source
AI summary
The present application provides a collagen type III composition prepared from a fibroblast extracellular matrix. The collagen type III is ultimately prepared by further purification, detection, and isolation based on the extracellular matrix components in a conditioned culture solution for fibroblasts, which are the most important cells in the human skin dermis, and can be combined with hyaluronic acid to form a composition formulation for use by people who want to remove and fill wrinkles on face and neck. The method is a production technique of the collagen type III composition from the fibroblast extracellular matrix, which belongs to a transplantation technology. More specifically, the human collagen is prepared from the cell culture supernatant by in vitro culturing the skin donated by volunteers for fibroblasts. The detection results of HIV, HBV, HCV, and syphilis infection markers are all non-reactive, mycoplasma is negative, and bacterial detection is negative.