Electrode for enzymatic biosensor with fibrous material, method of preparation thereof and said biosensor
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Solution Overview
Problem
Existing enzymatic biosensors face challenges in efficiently detecting analytes due to diffusion limitations in biofilms, leading to reduced sensitivity and limited enzyme availability, and three-dimensional electrodes are rigid and difficult to agitate or expel liquid samples.
Innovation Solution
A fibrous electrode for enzymatic biosensors is developed, comprising compressible and resilient conductive and enzyme-functionalized fibers, allowing for modifiable liquid sample quantity, easy agitation, and expulsion, while maintaining efficient enzyme-analyte interaction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a biofilm containing enzymes is used on a planar electrode, then the sensor can detect analytes, but the diffusion of substrate and reaction products is limited, reducing sensitivity
Solution Approach 1:
The invention transitions from a planar two-dimensional electrode to a three-dimensional fibrous electrode structure. This dimensional change increases the surface area and volume available for enzyme immobilization, allowing greater enzyme quantity and improved substrate diffusion pathways throughout the three-dimensional network, thereby resolving the contradiction between detection sensitivity and enzyme availability.
Solution Approach 2:
The fibrous electrode structure inherently creates a porous network with interconnected voids and channels. This porous architecture facilitates enhanced diffusion of substrate and reaction products throughout the electrode volume while providing extensive surface area for enzyme immobilization, simultaneously improving both detection sensitivity and enzyme availability.
2Measurement precision
If the thickness of the enzyme-containing layer is reduced to increase contact between reaction product and electrode, then diffusion efficiency improves, but the quantity of available enzymes is limited
Solution Approach 1:
By transitioning to a three-dimensional fibrous structure, the electrode maintains short diffusion distances between enzymes and conductive surfaces throughout the volume, eliminating the need to reduce layer thickness. The 3D architecture provides extensive internal surface area accessible to diffusing species, allowing both high sensitivity and extended sensor usage range through increased enzyme quantity.
3Productivity
If a three-dimensional electrode is used to contain liquid sample, then quantitative reactions can be performed, but the electrode is rigid and difficult to agitate or expel liquid
Solution Approach 1:
The fibrous electrode structure functions as a flexible, compressible matrix rather than a rigid container. This flexible architecture allows the electrode to be easily compressed to expel liquid samples and manipulated for agitation, while still maintaining its three-dimensional structure for containing the liquid sample and performing quantitative reactions.
4Reliability
If a rigid three-dimensional electrode is used, then liquid sample can be contained, but the electrode is difficult to compress or deform for sample handling
Solution Approach 1:
The flexible fibrous structure allows the electrode to be compressed and deformed to modulate the volume of liquid sample contained within. This adaptability enables the electrode to accommodate different sample quantities while maintaining reliable containment through its three-dimensional network structure.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The fibrous electrode enhances the sensitivity and efficiency of analyte detection by facilitating better enzyme-analyte contact and allowing for sequential measurements, while its compressibility and resilience simplify the handling of liquid samples.
Implementation Method 1
a conductive electrode enabling the electrochemical reaction or/and the transfer of electrons to a measurement electronic
Implementation Method 2
one or more enzymes making it possible to transform the analyte to assay (here glucose) into a reaction product measurable by an electrochemical reaction
Implementation Method 3
The latter must penetrate into the biofilm to be brought into contact with the enzymes
Data Source
AI summary
An electrode for enzymatic biosensor being in the form of a fibrous material and comprising electrically conductive fibres and electrically non-conductive fibres, all or part of which are functionalised by enzymes, identical or different. A method for preparing such an electrode and an electrochemical detection enzymatic biosensor comprising same.