Filter Suction Holder for Nucleic Acid Extraction
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Solution Overview
Problem
Conventional nucleic acid extraction methods face difficulties in handling and inserting a thin membrane filter into a reaction tube due to its tendency to stick to the tube's inner wall, complicating the process and increasing the risk of contamination.
Innovation Solution
A filter suction holder with a container that adheres to the filter surface, allowing easier insertion into a reaction tube, and optionally includes a heat-resistant or meltable material to enhance contact with a surface-active agent for improved extraction efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If the membrane filter is picked up with sterilized tweezers and inserted into the reaction tube, then the filter can be transferred to the reaction tube, but the thin filter tends to stick to the tube's inner wall, complicating the process and increasing contamination risk
Solution Approach 1:
A holder is introduced as an intermediary device between the filter and the reaction tube. The holder has a container that adheres to the filter surface and facilitates insertion into the reaction tube, preventing the filter from directly contacting and sticking to the tube wall, thereby reducing contamination risk while easing the insertion process
2Productivity
If the filter is directly inserted into the reaction tube, then the process is simple, but the filter sticks to the inner wall making insertion difficult and complicated
Solution Approach 1:
The holder merges multiple functions into a single device: it holds the filter, facilitates insertion into the reaction tube, and prevents sticking. This integration improves productivity by ensuring reliable filter placement while managing the complexity through a unified structure rather than separate操作步骤
3Ease of operation
If the filter surface has limited contact area with the reaction solution, then insertion is easier, but nucleic acid extraction efficiency is reduced
Solution Approach 1:
The holder's container is designed with specific local properties: the inner wall surface has adhesion characteristics that allow it to stick to the filter surface. This localized adhesion quality enables the container to securely hold the filter during insertion while allowing the filter to subsequently make adequate contact with the reaction solution for efficient nucleic acid extraction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Facilitates easier and more reliable insertion of the filter into the reaction tube, reducing contamination risk and enhancing nucleic acid extraction efficiency by increasing contact area with the reaction solution.
Implementation Method 1
the container having an inner wall surface configured to stick to a surface of the filter and cause the filter to be absorbed
Data Source
AI summary
A filter suction holder is insertable into a reaction tube together with a filter. The filter is configured to capture a specimen having nucleic acid by filtering a sample containing the specimen. The reaction tube is to be used for extracting the nucleic acid from the specimen. The filter suction holder includes a container for containing the filter. The container has an inner wall surface configured to stick to a surface of the filter and cause the filter to be absorbed.