Filtration Holder Structure for Gentle Cell Recovery
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Solution Overview
Problem
Existing filtration systems face difficulties in easily recovering filtration objects, particularly cells, due to challenges in separating them from filters without causing damage and inefficiencies in recovery rates.
Innovation Solution
A filtration recovery device and method involving a filtration filter with through-holes and a holder having an introduction and discharge port, where the filter is positioned between these ports, allowing for the capture and recovery of filtration objects by immersing them in a recovery liquid, with features like projection portions and adjustable liquid amounts to facilitate easy recovery.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If a conventional cell strainer with filtering portion on side surface and bottom surface is used, then filtration can be performed, but recovery of filtration object becomes difficult
Solution Approach 1:
The device is divided into distinct functional segments: a holder body, a detachable filtration filter, and a recovery chamber. This segmentation allows the filtration filter to be easily removed and the recovery chamber to be accessed separately, making filtration object recovery straightforward without complicating the overall filter structure.
Solution Approach 2:
A recovery chamber is introduced as an intermediary space between the filtration filter and the external environment. This chamber temporarily holds the filtration objects after filtration, allowing them to be recovered easily without direct manipulation of the filter structure itself, thus simplifying the recovery process.
2Productivity
If filtration filter is disposed in flow path between introduction port and discharge port, then liquid flow is enabled, but physical stress on filtration object increases
Solution Approach 1:
The device changes the physical parameters of the liquid by introducing a recovery liquid into the recovery chamber. This recovery liquid creates a cushioning effect that reduces physical stress on filtration objects during the transfer and recovery process, while maintaining high filtration efficiency through the properly positioned filtration filter in the flow path.
3Ease of operation
If discharge port is closed and recovery liquid is introduced from introduction port, then filtration object recovery is facilitated, but device complexity increases
Solution Approach 1:
The holder structure is designed with multi-functionality: the same introduction port and discharge port are used for both filtration operation and recovery liquid introduction. The holder body serves both as a support structure for the filtration filter and as a containment vessel for the recovery chamber. This universal design facilitates easy recovery without significantly increasing device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient and damage-free recovery of filtration objects, such as cells, by reducing physical stress and improving recovery rates through controlled liquid immersion and positioning of the filter within the holder.
Implementation Method 1
a filtration filter having a plurality of through-holes
Data Source
AI summary
A filtration recovery method for filtering and recovering a liquid containing a filtering object that includes: sinking a filtration recovery device including a filtration filter having a plurality of through-holes, an inlet for introducing the liquid, an outlet for discharging the liquid, and a holder having a flow path communicating the inlet and the outlet, and the filtration filter is arranged in the flow path, into the liquid containing the filtering object contained in a liquid storage container with one end side of the holder provided with the outlet so as to capture the filtering object with the filtration filter; introducing the liquid from the outlet of the holder of the filtration recovery device; closing the outlet of the holder of the filtration recovery device; and recovering the filtering object together with the liquid held in the holder of the filtration recovery device.