Fish Allergen Antigen Panels for Trace IgE Detection
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Solution Overview
Problem
Conventional allergy tests for fish are inefficient in detecting IgE antibody reactions to trace allergen components, leading to low diagnostic accuracy and inability to predict allergic reactions accurately.
Innovation Solution
Identification of novel antigens such as Alpha-actinin-3, EEF1A2 binding protein-like, Alpha-1,4-glucan phosphorylase, Elongation factor 2, Heat shock cognate 70 kDa protein, Serotransferrin, Myosin binding protein H-like, Desmin, Capping protein (Actin filament) muscle Z-line beta, Myosin heavy chain, Glycogen phosphorylase, Myosin-binding protein C, ATP synthase subunit beta, and L-lactate dehydrogenase A chain, which specifically bind to IgE antibodies in fish-allergic patients, enabling precise diagnosis and treatment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional allergy testing agents containing multiple proteins are used, then the test can detect positive allergic reactions when allergen components are present in sufficient amounts, but the diagnostic efficiency is insufficient when allergen components are present in trace amounts
Solution Approach 1:
The patent segments the complex fish allergen mixture into individual purified protein components (parvalbumin, tropomyosin, myosin heavy chain, actin, aldolase, enolase). By separating and identifying specific allergenic proteins, the test can detect trace amounts of individual allergens rather than requiring high concentrations of mixed proteins, thereby improving measurement precision for low-level allergen detection.
Solution Approach 2:
The patent extracts and purifies specific allergenic proteins from fish sources through protein extraction and purification processes. This extraction of individual allergen components (such as parvalbumin at 12 kDa, tropomyosin at 33 kDa) from the complex fish protein mixture enables the development of targeted diagnostic reagents with high sensitivity for detecting trace allergen levels in patient sera.
2Ease of manufacture
If conventional allergy tests use ground food materials containing numerous proteins, then the preparation is simple, but the content of each individual protein is very little making positive reaction determination difficult
Solution Approach 1:
Instead of using ground food materials as a mixture, the patent segments the complex protein mixture into individual purified allergen components. Each purified protein (parvalbumin, tropomyosin, etc.) can be prepared as a separate standardized reagent, improving both manufacturing clarity and diagnostic precision while maintaining systematic preparation approaches.
Solution Approach 2:
The patent changes the physical and chemical parameters of allergen preparation by purifying proteins to specific concentrations and molecular weight ranges (e.g., parvalbumin at 12 kDa, tropomyosin at 33 kDa). This parameter optimization ensures each reagent contains sufficient concentrations of specific allergens for reliable detection, transforming the approach from crude extracts to standardized purified protein reagents.
3Reliability
If fish allergen components are not specifically identified, then the allergy test may miss trace allergen reactions, but exhaustive identification of all allergen components increases test complexity
Solution Approach 1:
The patent segments the fish allergen profile into major recognized components (parvalbumin, tropomyosin, myosin heavy chain, actin, aldolase, enolase) that can be tested individually. This segmentation allows comprehensive allergen identification without requiring analysis of every possible protein, balancing diagnostic reliability with manageable test complexity through focused analysis of known major allergens.
Data Source
AI summary
The present invention provides novel antigens of an allergy to fish, methods and kits for diagnosing an allergy to fish, a pharmaceutical composition comprising the antigen, fish or fish eggs in which the antigen is eliminated, a processed product of the fish or the fish eggs or fish which lay the fish eggs or have hatched from the eggs, and a tester for determining the presence or absence of a fish antigen in an object of interest.