Fish DNA Extract Processing for Osteoarthritis and Bone Loss
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
There is a need for novel DNA extracts from fish and development of medical applications of such DNA extracts, particularly for treating osteoarthritis and osteoporosis, as well as addressing oxidative stress and promoting black hair formation.
Innovation Solution
A process is developed to extract DNA from fish testes, specifically using deep sea fish like mackerel or salmon, involving freezing, homogenization, acid treatment, centrifugation, pH adjustment, heating, and enzyme treatment to obtain a DNA extract with specific base pair lengths, which is then administered orally for therapeutic effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If DNA is extracted from salmon sperm, then PDRN product is obtained with tissue repairing and anti-inflammatory activities, but the DNA molecular weight is limited to 50-2000 bp which restricts bone regeneration capability
Solution Approach 1:
The patent segments the DNA extraction process by using multiple enzymes (proteinase K, collagenase, chondroitinase ABC) to selectively digest different components, thereby obtaining DNA fragments within the specific molecular weight range of 50-2000 bp suitable for PDRN product while maintaining the desired biological activities
Solution Approach 2:
The patent changes the molecular weight parameter of DNA by controlling enzyme digestion conditions and extraction parameters to produce DNA fragments in the 50-2000 bp range, which resolves the contradiction between achieving tissue repairing activities and obtaining the appropriate DNA size for PDRN formulation
2Reliability
If DNA disks are implanted into bone defects, then osteogenic cell migration and new bone formation are induced, but the DNA disks are biodegraded within 3 days which limits sustained therapeutic effect
Solution Approach 1:
The patent applies preliminary action by pre-treating the DNA with specific enzymes to generate fragments of optimal size (50-2000 bp) that can effectively induce osteogenic cell migration and bone formation while being designed for controlled release over time, addressing both the bone regeneration efficacy and the limited persistence duration
Solution Approach 2:
The patent introduces dynamics by creating a DNA product that dynamically releases therapeutic fragments in the body, where the enzymatically processed DNA maintains stability long enough to exert therapeutic effect but eventually degrades to avoid long-term persistence, balancing bone regeneration capability with appropriate duration of action
3Reliability
If PRF001 is administered orally, then anti-inflammatory effects and protective effects against osteoarthritis are achieved, but the molecular weight range of 50-1500 kDa may not optimize therapeutic efficacy
Solution Approach 1:
The patent optimizes the molecular weight parameter by using controlled enzymatic digestion to produce DNA fragments specifically in the 50-2000 bp range, which is more precise than the conventional 50-1500 kDa range, thereby enhancing the protective effect against osteoarthritis while improving manufacturing precision
Solution Approach 2:
The patent replaces mechanical fragmentation methods with enzymatic digestion using specific enzymes (proteinase K, collagenase, chondroitinase ABC) to achieve precise control over DNA fragment size distribution, substituting a chemical-biological system for mechanical processing to obtain more uniform and therapeutically optimized molecular weight ranges
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The DNA extract effectively reduces osteoarthritis pain, inflammation, bone resorption, and promotes black hair formation, while also reducing oxidative stress and improving bone density, demonstrating therapeutic potential for osteoporosis.
Implementation Method 1
The fish testes are frozen and thawed before the homogenization step
Implementation Method 2
the suspension is heated. In a preferred embodiment, the heating is 60-150° C., preferably 70-130° C. for 5-150 min
Implementation Method 3
the homogenate is subjected to centrifugation to collect a pellet
Data Source
AI summary
The present disclosure provides a method of extracting DNA from fish and a method of using the DNA extract. The DNA extract provided herein produces the efficacies in ameliorating or treating osteoarthritis or osteoporosis. Mice experiments prove that clinical parameters associated with osteoarthritis or osteoporosis are improved by the DNA extract. In addition, the DNA extract exhibits free radical scavenging activities, anti-oxidative activities, anti-depressant activities, and black hair promoting activities.