Fish Scale Biomaterial Acellularization for Tissue Repair

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Solution Overview

Problem

Current biomaterials like collagen fiber, hydroxyapatite, and tri-calcium phosphate have low mechanical strength and risk of terrestrial disease transmission, limiting their effectiveness in tissue repair and implant applications.

Innovation Solution

A tissue repair material is developed from acellularized fish scales, processed to remove albumin and glycosaminoglycans, and extruded into various forms under controlled temperatures to maintain mechanical strength and reduce disease transmission risks.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If collagen fiber, hydroxyapatite, and tri-calcium phosphate are used as biomaterials, then biocompatibility and safety are improved, but mechanical strength deteriorates

Engineering Contradiction:
ImprovebiocompatibilityVSAvoidmechanical strength
Core Design Contradiction:
ReliabilityVSStrength

Solution Approach 1:

The patent uses fish scale as a composite material that naturally contains both collagen fibers and hydroxyapatite minerals in their native configuration. This composite structure preserves the high mechanical strength of the natural material while maintaining biocompatibility, overcoming the limitation of using isolated biomaterial components separately

Inventive Principle:
Principle #40Composite materials

2Reliability

If conventional biomaterials are used, then biocompatibility is improved, but risk of terrestrial disease transmission increases

Engineering Contradiction:
ImprovebiocompatibilityVSAvoiddisease transmission risk
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and utilizes fish scale material as an alternative source that is free from terrestrial animal pathogens. By sourcing material from fish rather than terrestrial animals, the invention eliminates the disease transmission risk while preserving the beneficial biomaterial properties

Inventive Principle:
Principle #2Taking out (Extraction)

3Object-affected harmful factors

If fish scale is acellularized to remove albumin and glycosaminoglycans, then disease transmission risk is reduced, but manufacturing complexity increases

Engineering Contradiction:
Improvedisease transmission riskVSAvoidmanufacturing complexity
Core Design Contradiction:
Object-affected harmful factorsVSDevice complexity

Solution Approach 1:

The patent performs acellularization as a preliminary treatment step before extrusion and forming. By removing albumin and glycosaminoglycans in advance, the material is prepared for subsequent processing while eliminating disease transmission risks. This preliminary action simplifies the overall manufacturing workflow by addressing safety concerns early in the process

Inventive Principle:
Principle #10Preliminary action

4Strength

If fish scale is extruded under controlled temperature, then mechanical strength is maintained, but energy consumption increases

Engineering Contradiction:
Improvemechanical strengthVSAvoidenergy consumption
Core Design Contradiction:
StrengthVSUse of energy by moving object

Solution Approach 1:

The patent employs controlled temperature extrusion as a parameter change strategy to maintain the mechanical properties of fish scale during processing. By optimizing the extrusion temperature within a specific range, the invention preserves the native structure and strength of the material while minimizing energy input requirements

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The resulting biomaterial retains high mechanical strength, reduces the risk of terrestrial disease transmission, and is suitable for tissue repair and implant applications while maintaining the original bonding and 3D structure of fish scales.

Implementation Method 1

acellularizing a fish scale; and extruding the fish scale in a desired mold for forming a flaky form, a lump form or a specific form after acellularizing the fish scale to remove most albumin and few glycosaminoglycans

Methodology Applied
Scientific EffectAcellularization:

Implementation Method 2

The step of dehydrating the fish scale is performed until the fish scales containing less than about 50% of water

Methodology Applied
Scientific EffectDehydration: Desiccation

Implementation Method 3

the method can comprise grinding the fish scale and before the step of grinding the fish scale, further comprise a step of dehydrating the fish scale

Methodology Applied
Scientific EffectGrinding: Abrasion

Implementation Method 4

extruding the fish scale for forming a flaky form, a lump form or extruding in a desired mold for forming a specific form after acellularizing the fish scale

Methodology Applied
Scientific EffectExtrusion: Extrusion

Data Source

PatentEP3329944B1A method for preparing a biomaterial
Publication Date: 2019.12.25 BODY ORGAN BIOMEDICAL CORP
  • EP3329944B1 patent drawingFigure 1A
  • EP3329944B1 patent drawingFigure 1B
  • EP3329944B1 patent drawingFigure 1C

AI summary

A biomaterial prepared from a process comprising acellularizing the fish scales, dehydrating the fish scales until the fish scales containing less than about 50% of water, and grinding the dehydrated fish scales into ground particles each having an average size of less than about 10,000 µm in diameter, wherein the ground particles contain a mixture of sponge like matrix and powder is provided. The invention also provides a biomaterial prepared from fish scales by a process comprising subjecting the fish scales to a heat treatment at a temperature of less than about 200°C.