Fish-Testis PDRN Exosomes via Ultrasonication for Cellular Uptake
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Solution Overview
Problem
Existing methods struggle to produce exosomes in large quantities, particularly those containing polydeoxyribonucleotide (PDRN), which are essential for therapeutic applications due to their potential in tissue regeneration and anti-inflammatory effects.
Innovation Solution
A method involving the isolation and ultrasonication of fish testis tissue to produce high-purity, low-molecular weight PDRN-containing exosomes, utilizing specific frequency and intensity conditions to enhance yield and efficacy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional cell culture methods are used to produce exosomes, then the purity of exosomes can be maintained, but the production quantity is insufficient for clinical use
Solution Approach 1:
The patent uses fish testis tissue as a disposable, non-living biological material source instead of maintaining living cell cultures. The tissue is processed once and then discarded, eliminating the need for complex, ongoing cell culture maintenance while enabling large-scale exosome production. This approach transforms a sustainable but low-yield cell culture system into a high-yield batch processing system using readily available biological material.
Solution Approach 2:
The patent changes the source material parameters from cultured mammalian cells to fish testis tissue, which naturally contains high concentrations of PDRN. This parameter change in the biological source enables simultaneous achievement of high exosome yield and high PDRN content, overcoming the limitation of conventional methods that require extensive cell cultivation infrastructure.
2Manufacturing precision
If exosomes are produced from fish testis tissue without ultrasonication, then the production process is simpler, but the molecular weight of PDRN remains high reducing efficacy
Solution Approach 1:
The patent applies ultrasonic vibration to the fish testis tissue during processing. This mechanical vibration energy breaks down the tissue structure and shears the PDRN into lower molecular weight fragments. The ultrasonication step is straightforward to implement and directly achieves the desired PDRN molecular weight reduction without requiring complex enzymatic digestion protocols or multiple processing steps.
3Reliability
If high molecular weight PDRN is used in exosomes, then the structural integrity is maintained, but the intracellular uptake efficiency is reduced
Solution Approach 1:
The patent deliberately changes the molecular weight parameter of PDRN from high to low through ultrasonication. This parameter modification enables the PDRN to be more efficiently taken up by cells while still maintaining its functional integrity within the exosome structure. The lower molecular weight PDRN fragments can penetrate cell membranes more effectively, achieving the desired reliability improvement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method significantly increases the production of high-purity, low-molecular weight PDRN exosomes, improving their intracellular uptake and efficacy for tissue regeneration and anti-inflammatory compositions.
Implementation Method 1
providing ultrasound stimulation to fish testis tissue
Implementation Method 2
providing ultrasound stimulation to fish testis tissue
Data Source
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AI summary
The present invention relates to polydeoxyribonucleotide (PDRN)-containing exosomes, a composition including same, and a preparation method therefor, wherein the PDRN-containing exosomes of the present invention are derived from a fish testis tissue and include high-purity, low-molecular weight PDRN. In addition, exosomes containing a significant amount of low molecular weight PDRN can be induced through a simple process of ultrasonic treatment. Furthermore, application of ultrasound to the separated exosomes can prepare PDRN exosomes that are made to have low molecular weights, whereby the exosomes exhibit high intracellular uptake rates and thus can be advantageously used for manufacturing a tissue regeneration or anti-inflammatory composition.