Fish Tissue Perfusion for Live Preservation
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Solution Overview
Problem
Preserving fish tissue during transportation is challenging due to tissue damage or death from lack of oxygen and nutrients, leading to inferior products, and existing methods like freezing or transporting in oxygenated water are inefficient and costly.
Innovation Solution
A method involving perfusion of a fluid through the circulatory system of fish using a needle to maintain tissue viability, which includes using a fluid with nutrients and antioxidants to reduce stress and prevent tissue damage, allowing for extended transportation times without extreme cooling.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If freezing is used to preserve fish tissue during transportation, then tissue viability is maintained, but cell death and tissue damage occur leading to inferior product quality
Solution Approach 1:
The patent changes the physical state parameters of the preservation medium from frozen solid to liquid perfusate at physiological temperature. The perfusion fluid contains modified constituents including antioxidants, anti-apoptotic agents, and anti-inflammatory compounds that alter the chemical composition parameters to prevent cell death while maintaining tissue viability during transport.
Solution Approach 2:
The patent introduces a perfusion fluid as an intermediary substance that mediates between the fish tissue and the external environment. This fluid acts as a carrier delivering protective agents directly to the tissue, preventing harmful effects of transport while maintaining physiological conditions without requiring freezing.
2Quantity of substance
If live fish are transported in tanks full of water sparged with oxygen, then oxygen supply is improved, but physical damage occurs and transport weight increases leading to higher costs
Solution Approach 1:
The patent extracts the fish from the aquatic environment and isolates the specific physiological system (circulatory system) that requires oxygen. Instead of oxygenating the entire water environment, oxygen and other protective agents are delivered directly through the circulatory system via perfusion, eliminating the need for heavy water tanks while preventing physical damage.
Solution Approach 2:
The patent uses hydraulic perfusion to deliver oxygen-containing fluid through the fish circulatory system. A pump system creates fluid pressure to circulate the oxygenated perfusate through vessels, providing efficient oxygen delivery without requiring large volumes of water or sparging equipment.
3Quantity of substance
If live fish are transported in tanks full of water sparged with oxygen, then oxygen availability increases, but transport weight increases and capacity decreases
Solution Approach 1:
The patent extracts only the essential oxygen delivery function from the water tank system. By perfusing oxygen directly through the circulatory system, the method eliminates the need to transport heavy water tanks while maintaining adequate oxygen supply to the fish tissue during transport.
4Duration of action of moving object
If traditional transportation methods are used, then transport time is limited, but tissue breakdown accelerates leading to inferior products
Solution Approach 1:
The patent applies preliminary protective action by perfusing the fish tissue with antioxidants, anti-apoptotic agents, and anti-inflammatory compounds before and during transport. This pre-protection prevents tissue breakdown from occurring during the extended transport period, enabling longer duration transport without quality degradation.
Solution Approach 2:
The patent establishes continuous perfusion of protective fluids throughout the transport period. The circulatory system remains actively perfused with oxygenated, nutrient-containing, and protective agent-laden fluid continuously, maintaining tissue viability and preventing breakdown over extended durations compared to intermittent or static preservation methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method improves the quality and freshness of fish tissue by reducing tissue breakdown, enabling longer transportation durations and lower costs compared to traditional methods, while maintaining tissue viability and quality.
Implementation Method 1
perfusing a circulatory system of one or more fish with a fluid mixture
Implementation Method 2
The fluid mixture supplies every tissue of the fish with nutrients and gasses
Data Source
AI summary
The present disclosure describes systems, devices, compositions, and methods for live tissue preservation.