Fixative Stabilization for Dynamic Gene Expression Monitoring
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Solution Overview
Problem
Current methods for analyzing gene expression are limited by the degradation of mRNA and the use of preservative compositions that cause irreversible damage to nucleic acids, making it difficult to obtain high-quality RNA for clinical and research purposes.
Innovation Solution
The method involves obtaining biological samples at multiple time points, stabilizing them using fixative agents like formaldehyde or alcohol to prevent degradation, and quantifying polynucleotides or polypeptides to determine changes indicative of mental, emotional, or physical states, using a system that includes a computer program for data analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If preservative compositions are used to maintain biological samples, then sample stability is improved, but nucleic acid quality deteriorates due to irreversible damage
Solution Approach 1:
The patent applies preliminary action by stabilizing biological samples immediately upon collection using fixative agents like formaldehyde or alcohol. This prevents mRNA degradation before analysis, allowing high-quality RNA to be obtained without requiring preservative compositions that would cause irreversible damage to nucleic acids.
2Loss of information
If mRNA is extracted from biological samples for gene expression analysis, then gene expression data is obtained, but mRNA degradation occurs reducing analysis accuracy
Solution Approach 1:
The patent applies preliminary anti-action by using fixative agents to prevent mRNA degradation before extraction and analysis. This counteracts the natural degradation process, preserving mRNA integrity and ensuring accurate gene expression analysis without information loss.
3Measurement precision
If multiple time point sampling is performed for dynamic monitoring, then state change detection is improved, but sample collection complexity increases
Solution Approach 1:
The patent uses fixative agents as intermediaries that can be applied to biological samples during routine collection procedures. This allows multiple time point sampling for dynamic monitoring without significantly increasing system complexity, as the fixative agents integrate seamlessly into existing sampling workflows while enabling precise state change detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the effective monitoring of mental, emotional, or physical states by preventing mRNA degradation and maintaining cellular integrity, enabling accurate gene expression analysis and providing a non-invasive, efficient means for disease diagnosis and prognosis.
Implementation Method 1
stabilizing them using fixative agents like formaldehyde or alcohol to prevent degradation
Data Source
AI summary
This disclosure relates to methods and kits, systems for screening, diagnosing and prognosing a disease, disorder, or physiological state based upon temporal measurements and analysis of gene expression in a subject.