Flavonoid Inclusion Compound Production via Rhamnose Cleavage
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Solution Overview
Problem
Existing methods for producing flavonoid inclusion compounds and compositions of flavonoid glycosides are not efficient in improving solubility, leading to insufficient water solubility of flavonoids, particularly those with a rhamnoside structure.
Innovation Solution
A method involving the cleavage of rhamnose from sparingly soluble flavonoids using an enzyme with rhamnosidase activity in the presence of cyclodextrin, followed by glycosidation with a glycosyltransferase, to produce flavonoid inclusion compounds with enhanced water solubility, specifically targeting isoquercitrin, hesperetin-7-glucoside, and naringenin-7-glucoside.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a sparingly soluble flavonoid with a rhamnoside structure is used directly, then the physiological effects and bioavailability are limited, but the water solubility is insufficient for use in foods, beverages, medicaments, or cosmetics
Solution Approach 1:
The invention extracts and removes the rhamnose moiety from the rhamnoside structure of flavonoids using enzymatic hydrolysis with beta-glucosidase or other rhamnosidases, converting sparingly soluble flavonoid rhamnosides into more water-soluble aglycones or glucose conjugates that can be effectively used in aqueous formulations
Solution Approach 2:
The invention changes the chemical structure parameter of flavonoids by cleaving the rhamnoside bond, thereby transforming the solubility parameter from sparingly soluble to highly water-soluble, enabling effective use in foods, beverages, medicaments, and cosmetics
2Productivity
If existing methods for producing flavonoid inclusion compounds are used, then the production process is complex and inefficient, but the water solubility improvement is insufficient
Solution Approach 1:
The invention merges the hydrolysis reaction and inclusion compound formation into a single integrated process where cyclodextrin is present during the enzymatic cleavage of rhamnose, allowing simultaneous production of water-soluble flavonoid derivatives and their inclusion compounds, thereby simplifying the production process and improving both efficiency and solubility
Solution Approach 2:
The invention uses cyclodextrin as an intermediary substance that facilitates both the hydrolysis reaction and the formation of water-soluble inclusion compounds, acting as a mediator that enhances water solubility while streamlining the production process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high solubility of flavonoids in water, improving their bioavailability and physiological effects, and can be applied to foodstuffs, medicaments, and cosmetics, enhancing their functionality and stability.
Implementation Method 1
treating a sparingly soluble flavonoid having a rhamnoside structure with an enzyme having a rhamnosidase activity to cleave a rhamnose
Implementation Method 2
in the presence of a cyclodextrin to cleave a rhamnose... producing a water-soluble flavonoid and a cyclodextrin
Data Source
AI summary
A method for producing a flavonoid inclusion compound, including a cleaving step including treating a sparingly soluble flavonoid having a rhamnoside structure with an enzyme having a rhamnosidase activity in the presence of a cyclodextrin to cleave a rhamnose. According to the production method of the present invention, a flavonoid inclusion compound and a composition of flavonoid glycosides having excellent solubility in water can be efficiently produced, so that the compound and composition can be suitably utilized in the fields of medicaments, foodstuff, health foods, foods for specified health use, cosmetics, and the like.