Flexible Sheet Cassette with Fluorescent Chips for Cell Culture
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing biological component kits for cell culture in regenerative medicine face challenges in efficient setup and accurate parameter detection, leading to potential mistakes and suboptimal product quality due to complex pathways and rigid cassette designs, which hinder the detection and adjustment of culture medium parameters in real-time.
Innovation Solution
A flexible sheet-shaped biological component cassette with integrated flow paths and fluorescent chips that change color in response to specific substances, allowing for real-time detection of parameters like oxygen, pH, glucose, and carbon dioxide, coupled with an optical sensor system for precise measurement and adjustment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If a rigid biological component cassette is used to integrate multiple paths, then the setup time is reduced, but it becomes difficult to detect the state of liquid flowing through the flow paths
Solution Approach 1:
The patent employs a flexible sheet-shaped cassette main body that allows for easy detection of liquid flow states through its deformable structure, while still integrating multiple flow paths to reduce setup time. The flexibility enables visual or tactile detection of liquid presence without requiring additional rigid structural components.
Solution Approach 2:
The patent incorporates fluorescent chips that undergo coloring changes in response to predetermined substances in the liquid, enabling easy detection of liquid flow parameters. This color change mechanism allows operators to monitor liquid flow status directly through the cassette without additional detection equipment.
2Difficulty of detecting and measuring
If structural components are provided on the exterior of the cassette for flow path detection, then liquid flow can be detected, but additional setting operations are required reducing working efficiency
Solution Approach 1:
The patent integrates the detection functionality directly into the cassette main body by incorporating fluorescent chips within the flow paths. This merging of detection function into the structure itself eliminates the need for separate exterior detection components and their associated setting operations, thereby maintaining working efficiency.
Solution Approach 2:
The cassette is designed to be self-detecting through the integrated fluorescent chips that automatically indicate liquid flow states. The system serves its own detection needs without requiring external detection components or additional setting operations, improving working efficiency.
3Adaptability or versatility
If multiple medical bags and bioreactor are used to form culture pathways, then cell culture functionality is achieved, but the pathway becomes complicated requiring more setup time and increasing mistake probability
Solution Approach 1:
The patent integrates multiple flow paths into a single sheet-shaped cassette structure, combining what would otherwise be separate medical bags and bioreactor connections into one unified component. This merging maintains the necessary cell culture functionality while dramatically simplifying the overall pathway structure and reducing setup complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient setup and stable product quality by facilitating easy detection and adjustment of culture parameters, improving the accuracy and detail of cell culture management.
Implementation Method 1
a chip configured to undergo coloring in response to a predetermined substance contained in the liquid
Implementation Method 2
an optical sensor configured to detect the parameter related to culturing of cells, by emitting measurement light toward the fluorescent chip and receiving excitation light from the chip
Data Source
AI summary
A biological component treatment system includes a biological component kit, and a biological component treatment device in which the biological component kit is set. The biological component kit includes tubes and a biological component cassette, the biological component cassette includes flow paths in the interior thereof, and is equipped with a cassette main body formed in a sheet shape that possesses flexibility. In the flow paths, there are provided target parameter detection parts that make it possible to detect parameters related to the culturing of cells, and the target parameter detection parts include chips that undergo coloring in response to a predetermined substance contained in a liquid. Further, the biological component treatment device includes optical sensors that detect parameters related to culturing of cells.