Flow Cell Resin Composition With Low Autofluorescence Curing
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Solution Overview
Problem
Biological arrays used in genetic sequencing and molecule detection suffer from undesirable autofluorescence due to photoinitiators, which interfere with signal-to-noise ratios and hinder the resolution of individual cluster signals during sequencing.
Innovation Solution
A resin composition comprising a free radical curable resin matrix with acrylate and siloxane, and a free radical photoinitiator, which minimizes autofluorescence by avoiding intramolecular interactions between different photoinitiators, resulting in low or no autofluorescence when exposed to blue and green excitation wavelengths.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If photoinitiators are used in resin composition for flow cells, then the resin can be cured to form structural components, but autofluorescence increases which interferes with signal-to-noise ratios in sequencing
Solution Approach 1:
The patent extracts and removes traditional photoinitiators from the resin composition. Instead, it uses a photopolymerizable compound that can self-initiate polymerization upon light exposure without requiring separate photoinitiator additives, thereby eliminating the source of autofluorescence while maintaining curing functionality
Solution Approach 2:
The patent changes the chemical parameters of the resin system by selecting specific photopolymerizable compounds with appropriate absorption spectra and reactivity. The compound is designed to absorb at wavelengths that do not cause autofluorescence in the detection range, and to undergo efficient polymerization to form the cured resin structure
2Reliability
If traditional photoinitiators are used in the resin, then curing can be achieved, but intramolecular interactions between photoinitiators cause increased autofluorescence that hinders resolution of individual cluster signals
Solution Approach 1:
The patent removes traditional photoinitiators from the system entirely. The photopolymerizable compound serves dual functions as both the resin component and the light-sensitive initiator, eliminating the harmful intramolecular interactions that occur between separate photoinitiator molecules
Solution Approach 2:
The patent creates a composite resin system where the photopolymerizable compound is integrated into the resin matrix at the molecular level. This unified structure ensures that the curing process occurs without the formation of separate photoinitiator aggregates that would generate autofluorescence and interfere with signal resolution
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resin composition achieves significantly reduced autofluorescence, enabling high signal-to-noise ratios that allow for clear resolution of individual cluster signals during sequencing, with fluorescence levels below detection thresholds or minimal interference.
Implementation Method 1
a free radical curable resin matrix including an acrylate and a siloxane; and a free radical photoinitiator
Implementation Method 2
when cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm
Data Source
AI summary
An example of a resin composition includes a free radical curable resin matrix including an acrylate and a siloxane, and a free radical photoinitiator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.