Flow Cell Spectrophotometer Integrated in Liquid Handler

The patent combines the spectrophotometer and liquid handler into a single integrated apparatus. The flow cell spectrophotometer is positioned within the liquid handler body, allowing optical measurements to be performed during liquid handling operations. This merging eliminates the need for separate apparatus and improves compatibility with automated systems while maintaining functional optimization through dedicated components for each function.

The patent employs a flow cell spectrophotometer that allows dynamic sampling and measurement. The system can aspirate small volumes of sample (3-5 µL) into the flow cell for measurement, then dispense the same sample back into the original container for reuse. This dynamic approach enables multiple measurements from the same small sample volume, reducing sample consumption while maintaining accuracy through controlled flow through the optical path.

The patent creates a universal integrated apparatus where the flow cell spectrophotometer can be incorporated into automated liquid handlers. The system performs multiple functions: liquid handling, optical measurement, and sample reuse capability. This multi-functional design achieves high-throughput analysis capability while simplifying integration compared to plate reader systems, as the flow cell can be directly positioned within the liquid handler body without requiring complex plate mounting and positioning mechanisms.

The patent implements sample recovery through the flow cell spectrophotometer system. After optical measurement, the sample in the flow cell is dispensed back into the original container rather than being discarded. This recovery mechanism allows the minimum required sample volume for accurate readings (3-5 µL) to be reused for subsequent measurements or other experimental purposes, thereby maintaining both reading accuracy and sample availability.