Flow Cell Target Capture Beads for Focused DNA Enrichment
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Solution Overview
Problem
Existing sequencing techniques struggle to efficiently enrich and analyze specific genetic variants within a DNA sample, as they often require analyzing the entire sample, which can lead to unnecessary data and resource wastage.
Innovation Solution
The use of target capture beads with complementary capture probes in an enrichment channel of a flow cell, allowing for on-flow cell hybridization and capture of library fragments corresponding to the target portion, thereby enriching the region of interest.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the entire DNA sample is analyzed using existing sequencing techniques, then comprehensive genetic data is obtained, but data efficiency decreases and resource wastage increases due to analysis of non-relevant DNA segments
Solution Approach 1:
The patent extracts and isolates only the relevant target DNA sequences from the entire genome using capture probes that specifically bind to regions of interest. This extraction process separates the useful genetic information from the vast amount of non-relevant DNA, enabling focused sequencing that improves data efficiency and reduces resource consumption by eliminating unnecessary analysis of irrelevant genomic segments
Solution Approach 2:
The patent segments the genome into specific target regions of interest that can be individually captured and sequenced. By dividing the comprehensive genomic analysis into discrete, captureable segments, the system allows researchers to focus sequencing resources on particular genes or genomic regions, thereby improving measurement precision while reducing the energy and resources required for whole-genome sequencing
2Measurement precision
If target capture beads with complementary capture probes are used for enrichment, then focused sequencing reads are obtained for the target portion, but device complexity increases due to the enrichment channel and immobilization system
Solution Approach 1:
The patent introduces target capture beads as an intermediary component that mediates between the DNA sample and the sequencing process. These beads carry complementary capture probes that specifically bind to target sequences, acting as a bridge that enriches the desired genetic material before sequencing. This intermediary approach maintains high measurement precision while organizing the complexity into a modular, manageable system
Solution Approach 2:
The patent extracts the enrichment function into a separate, dedicated component (the target capture beads and enrichment channel), isolating the complexity of target selection from the main sequencing process. This extraction allows the core sequencing technology to remain relatively simple while the enrichment step handles the complexity of target identification and concentration
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables focused sequencing reads dedicated to the target portion, improving data efficiency and reducing unnecessary analysis of non-relevant DNA segments.
Implementation Method 1
capture probes including a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid
Data Source
AI summary
An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid.