Flow Cytometer Support Stage Feedback Alignment for Cell Sorting
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Solution Overview
Problem
Flow cytometers require substantial user input for setup and alignment, including manual adjustment of fluidics parameters and collection vessels, which can lead to inaccuracies in cell sorting.
Innovation Solution
A closed-loop feedback position encoder system for a support stage in a flow cytometer that adjusts position in the X-Y plane in response to data signals from a photodetector, enabling precise positioning and automated alignment of the support stage without manual input.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual alignment of flow stream and collection vessels is used, then device complexity is reduced, but positioning precision and sorting accuracy deteriorate
Solution Approach 1:
The patent implements a closed-loop feedback control system using encoders to continuously monitor the actual positions of the flow stream and collection vessels, comparing them with target positions, and automatically adjusting deviations. This feedback mechanism achieves high positioning precision without requiring overly complex manual alignment procedures, as the system self-corrects positioning errors through automated control loops.
Solution Approach 2:
The system performs self-alignment through automated encoder-based positioning and feedback control, eliminating the need for complex manual intervention. The flow cytometer automatically adjusts the relative positions of collection vessels and flow stream based on encoder data and control algorithms, enabling the device to service itself during setup and operation, thereby improving precision without proportionally increasing complexity.
2Manufacturing precision
If automated closed-loop feedback control is implemented, then positioning precision and sorting accuracy improve, but device complexity increases
Solution Approach 1:
The patent replaces complex mechanical alignment mechanisms with electronic encoder-based positioning and software-controlled feedback algorithms. Instead of using intricate mechanical adjustment devices, the system uses encoders to digitally track positions and computer-controlled actuators to make precise adjustments, substituting mechanical complexity with electronic and software-based solutions that achieve higher precision more efficiently.
Solution Approach 2:
The encoder system serves multiple functions simultaneously: it tracks position, provides feedback for control loops, enables automated alignment, and supports real-time adjustments during sorting operations. This multi-functionality reduces the need for separate dedicated components for each function, thereby improving sorting accuracy without proportionally increasing overall device complexity.
3Measurement precision
If real-time monitoring and correction of motion and position is performed, then positioning resolution and particle separation accuracy improve, but use of energy and computational resources increase
Solution Approach 1:
The system performs position monitoring and correction at periodic intervals determined by the encoder sampling rate and control loop frequency, rather than continuously. This periodic operation allows the system to maintain high positioning resolution while reducing energy consumption compared to truly continuous monitoring and correction, as the encoders and control systems can enter low-power states between measurement and adjustment cycles.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system provides precise positioning resolution, accurate particle separation, and reduces the need for user input, enhancing the accuracy and efficiency of cell sorting by continuously monitoring and correcting motion and position in real time.
Implementation Method 1
a photodetector configured to detect light from the irradiated particles
Implementation Method 2
As a cell moves through the interrogation point, it causes the laser light to scatter
Implementation Method 3
The laser light also excites components in the cell stream that have fluorescent properties
Implementation Method 4
the flow cell is rapidly vibrated by an acoustic device, such as a piezoelectric element
Data Source
AI summary
Aspects of the disclosure include systems for sorting particles of a sample in a flow stream (e.g., a biological sample containing cells). Systems according to certain embodiments include a flow cell configured to propagate a sample through a flow stream, a light source configured to irradiate particles of the sample in the flow stream, a photodetector configured to detect light from the irradiated particles and a support stage operationally coupled to the photodetector, where the support stage includes a closed-loop feedback position encoder that is configured to adjust position in the X-Y plane in response to a data signal generated by the photodetector in response to light from the irradiated particles. Methods for sorting particles using the subject systems are also described. Non-transitory computer readable storage medium are also provided.