Flow Cytometer Waveform Display with Color-Coded Sample Frequency
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Solution Overview
Problem
In optical detection of samples flowing through a flow path, it is challenging to intuitively recognize the frequency of samples, leading to inefficiencies in measurement and analysis accuracy due to the need for numerical data interpretation and complex waveform analysis.
Innovation Solution
An optical measuring apparatus that includes a light irradiating unit, a light detecting unit, and a rate information adding unit to display the sample frequency on a waveform graph, allowing for intuitive recognition through visual cues such as color or meter bars, and an optional optical axis adjusting unit for improved alignment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If numerical data interpretation and complex waveform analysis are used to determine sample frequency, then measurement precision is maintained, but ease of operation deteriorates due to the inability to intuitively recognize sample frequency
Solution Approach 1:
The patent applies color changes to the waveform display based on the sample frequency. Different colors are assigned to different frequency ranges, allowing operators to intuitively recognize the sample frequency at a glance without complex numerical analysis. This visual feedback mechanism maintains measurement precision while dramatically improving ease of operation.
2Ease of operation
If visual display of sample frequency is implemented, then ease of operation improves through intuitive recognition, but device complexity increases due to additional processing and display components
Solution Approach 1:
The patent implements multi-functionality by having the existing waveform display system serve dual purposes: traditional waveform visualization and sample frequency indication through color coding. This approach improves ease of operation without requiring entirely separate display systems, thereby limiting the increase in device complexity.
Solution Approach 2:
The patent changes the visual parameters of the existing waveform display (specifically color) to encode frequency information. This parameter change approach allows the system to convey additional information without adding substantial hardware complexity, as it leverages the existing display infrastructure.
3Measurement precision
If optical axis alignment is performed using traditional methods, then measurement precision can be maintained, but productivity deteriorates due to time-consuming adjustment procedures and higher abort rates
Solution Approach 1:
The patent implements feedback mechanisms that provide real-time information about sample frequency and alignment status to the operator. This feedback enables faster, more accurate optical axis alignment by allowing operators to make informed adjustments based on visual cues, thereby maintaining measurement precision while improving productivity through reduced adjustment time and lower abort rates.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables efficient and accurate measurement by intuitively displaying sample frequency, enhancing measurement efficiency and accuracy, and facilitating optical axis adjustments for improved alignment and reduced abort rates.
Implementation Method 1
microparticles are irradiated with a laser beam, and fluorescent light or scattered light emitted from each microparticle is detected
Implementation Method 2
microparticles are irradiated with a laser beam, and fluorescent light or scattered light emitted from each microparticle is detected
Implementation Method 3
fluorescent light or scattered light emitted from each microparticle is detected
Data Source
AI summary
Provided is an optical measuring apparatus including a light irradiating unit that irradiates a sample flowing through a flow path with light, a light detecting unit that detects optical information emitted from the sample due to light irradiation by the light irradiating unit, and a rate information adding unit that adds a predetermined display corresponding to a flow amount of the sample per unit time obtained from the optical information to a waveform data graph obtained from the optical information.